APOE antibody

Catalog No. ABIN965577

This anti-APOE antibody is a Mouse Monoclonal antibody detecting APOE in WB, IHC, ELISA and FACS. Suitable for Human. This Primary Antibody has been cited in 1 publication.

Quick Overview for APOE antibody (ABIN965577)

Target: APOE (Apolipoprotein E (APOE))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This APOE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Clone: 1H4

Product Details anti-APOE Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human ApoE expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes: Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-APOE antibody (ABIN965577)

Zintzaras, Kitsios, Triposkiadis, Lau, Raman: "APOE gene polymorphisms and response to statin therapy." in: The pharmacogenomics journal, Vol. 9, Issue 4, pp. 248-57, (2009) (PubMed).

Target Details for APOE

Target: APOE (Apolipoprotein E (APOE))

Alternative Name: ApoE

Background: Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptormediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants.
Tissue specificity: Occurs in all lipoprotein fractions in plasma. It constitutes 10-20% of very low density lipoproteins (VLDL) and 1-2% of high density lipoproteins (HDL). APOE is produced in most organs. Significant quantities are produced in liver, brain, spleen, lung, adrenal, ovary, kidney and muscle.
Synonyms: AD2, LPG, LDLCQ5, MGC1571

Molecular Weight: 36kDa

Gene ID: 348

Pathways: Regulation of Cell Size, Lipid Metabolism