TNNC1 antibody

Catalog No. ABIN965749

This Mouse Monoclonal antibody specifically detects TNNC1 in WB, ELISA, IHC (p) and IP. It exhibits reactivity toward Human.

Quick Overview for TNNC1 antibody (ABIN965749)

Target: TNNC1 (Cardiac Troponin C (TNNC1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNNC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Clone: 2D5

Product Details anti-TNNC1 Antibody

Specificity: This antibody is specific for human cardiac troponin subunit I, a 21 kD protein responsible for inhibiting actomyosin Mg2+-ATPase activity. This antibody has less than 0.1% reactivity with human skeletal TNI as measured by ELISA.

Characteristics: High temp. antigen demasking for FFPE tissue sections. Deparaffinize and rehydrate tissue sections.
10mM citrate buffer: pH 6.0. Boil 10-20 mins, followed by cooling 10-20 mins
OR Process tissue sections in Pressure cooker for 5 mins in citrate buffer followed by cooling 10-20 mins to RT. Wash tissue sections with buffer (PBS or Automation buffer,)

Purification: Concentrated.

Immunogen: Purified human cardiac troponin I.

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistochemistry:1:25. For formalin-fixed paraffin-embedded (FFPE) tissue sections antigen demasker (10 mM citrate buffer pH 6.0 ) is required. Boil the tissue 10-20 mins in this buffer, followed by cooling to RT.
Western blotting: 1:50-1:100.
ELISA: 1:500-1:1000
Immunoprecipitation.
Staining Procedure: This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:25-1:50 in IHC. When using paraffin-embedded and formalin-fixed tissues, high temperature antigenic unmasking technique is strongly recommended for consistent and reproducible results. This product can also be used on frozen tissue sections. The optimal conditions should be determined by the individual laboratory.

Comment:

Cellular Localization: cytoplasmic staining of cardiac muscle cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.4 mg/ml.

Buffer: 20 mM tris-borate, 150 mM Sodium Chloride, BMC-6 carrier polysaccharides, pH 7.5

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for TNNC1

Target: TNNC1 (Cardiac Troponin C (TNNC1))

Alternative Name: Cardiac Troponin-1

Molecular Weight: 21 kDa

Gene ID: 7134