CD247 antibody

Catalog No. ABIN965779

This Mouse Monoclonal antibody specifically detects CD247 in WB, ELISA, IHC, FACS and IF. It exhibits reactivity toward Human and has been mentioned in 3+ publications.

Quick Overview for CD247 antibody (ABIN965779)

Target: CD247 (CD247 Molecule (CD247))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD247 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 4B10

Product Details anti-CD247 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human CD247 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes: Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Immunofluorescence: 1/200 - 1/1000.
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-CD247 antibody (ABIN965779)

de Bakker, van Bodegom, van de Poll, Boelen, Nat, Rozema, Aerts: "Is UV-B radiation affecting charophycean algae in shallow freshwater systems?" in: The New phytologist, Vol. 166, Issue 3, pp. 957-66, (2005) (PubMed).

Nambiar, Fisher, Warke, Krishnan, Mitchell, Delaney, Tsokos: "Reconstitution of deficient T cell receptor zeta chain restores T cell signaling and augments T cell receptor/CD3-induced interleukin-2 production in patients with systemic lupus erythematosus." in: Arthritis and rheumatism, Vol. 48, Issue 7, pp. 1948-55, (2003) (PubMed).

Dumont, Blanchard, Di Bartolo, Lezot, Dufour, Jauliac, Hivroz: "TCR/CD3 down-modulation and zeta degradation are regulated by ZAP-70." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 4, pp. 1705-12, (2002) (PubMed).

Target Details for CD247

Target: CD247 (CD247 Molecule (CD247))

Alternative Name: CD247

Background: The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Synonyms: T3Z, CD3H, CD3Q, CD3Z, TCRZ, CD3-ZETA, CD247

Molecular Weight: 18kDa

Gene ID: 919

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway