CD31 antibody

Catalog No. ABIN965786

The Mouse Monoclonal anti-CD31 antibody has been validated for FACS, IHC and IP. It is suitable to detect CD31 in samples from Human. There is 1 publication available.

Quick Overview for CD31 antibody (ABIN965786)

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD31 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Clone: B493 (158-2B3)

Product Details anti-CD31 Antibody

Purification: Concentrated.

Immunogen: Human leucocytes

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry: 1:50-1:100.
Flow cytometry: 1:150-1:300
Immunoprecipitation.
Staining Procedure: This antibody can be used on frozen tissue sections. When using with formalin-fixed paraffin-embedded tissue sections an antigen demasking treatment is recommended. The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.

Comment:

Recommended Positive Control: Tonsil, Thymus, Spleen

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.3 mg/ml.

Buffer: 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-CD31 antibody (ABIN965786)

van Mourik, Leeksma, Reinders, de Groot, Zandbergen-Spaargaren: "Vascular endothelial cells synthesize a plasma membrane protein indistinguishable from the platelet membrane glycoprotein IIa." in: The Journal of biological chemistry, Vol. 260, Issue 20, pp. 11300-6, (1985) (PubMed).

Target Details for CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Alternative Name: CD31 (PECAM-1)

Gene ID: 5175

Pathways: Regulation of Actin Filament Polymerization