CD43A antibody

Catalog No. ABIN965797

This Mouse Monoclonal antibody specifically detects in IHC (fro), FACS and IF. It exhibits reactivity toward Human.

Quick Overview for CD43A antibody (ABIN965797)

Target: CD43A

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: B316 (DFT1)

Product Details

Specificity: This antibody reacts with a 95-115 kD protein on T-cells and thymocytes and a 115-135 kD molecules on neutrophils and platelets. These proteins are o-glycosylated and are mucin-like molecules with different antigenic epitopes.

Purification: Purified.

Immunogen: The myeloblast cell line KG1.

Isotype: IgG1 kappa

Application Details

Application Notes: Immunofluorescence: 10-20 ug/mL.
Flow cytometry: 1 ug/10^6 cells.
Immunoblotting: 1-5 ug/mL.
Immunohistochemistry: 5-10 ug/mL on frozen and formalin fixed sections using avidin-biotin system. This antibody was not quality controlled in flow cytometry.
Staining Procedure: This antibody can be used on frozen cryostat sections as well as formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation is buffered formalin can destroy the epitope. The antibody may be used at a dilution of 5-10 ug/mL in IHC. High temperature antigenic unmasking technique is recommended to enhance staining on formalin fixed tissue sections. It is recommended that this product be used on frozen tissue sections or specimens. The optimal conditions should be determined by the individual laboratory.

Comment:

Recommended Positive Control: Lymphoid Tissue sections

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.4 mg/ml.

Buffer: 50 mM Sodium Borate, 150 mM Sodium Chloride, 20% Glycerol, pH 8.0

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details

Target: CD43A