CD43B antibody

Catalog No. ABIN965798

The Mouse Monoclonal anti-CD43B antibody is suitable to detect CD43B in samples from Human. It has been validated for IHC (fp), IHC (fro), FACS and IF.

Quick Overview for CD43B antibody (ABIN965798)

Target: CD43B

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: B310 (BRA-7G)

Product Details

Specificity: This antibody reacts with a 95-115 kD glycoprotein on T-cells and thymocytes and a 115-135 kD molecule on neutrophils and platelets.

Purification: Purified.

Immunogen: Immature pluripotential human leukemia cell line K562.

Isotype: IgM

Application Details

Application Notes: Immunofluorescence: 5-20 ug/mL.
Flow cytometry: 1-5 ug/10^6 cells.
Westernblotting: 1-5 ug/mL.
Immunohistochemistry: 5-20 ug/mL on frozen sections using avidin-biotin system.
This product was not quality controlled in flow cytometry.
Staining Procedure: This antibody can be used on frozen cryostat sections as well as formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation is buffered formalin can destroy the epitope. The antibody may be used at a dilution of 5-20 ug/mL in IHC.

Comment:

Recommended Positive Control: Tonsil and Lymph Node

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.4 mg/ml.

Buffer: 50 mM Sodium Borate, 150 mM Sodium Chloride, 20% Glycerol, pH 8.0

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details

Target: CD43B