CD5 antibody

Catalog No. ABIN965806

This Mouse Monoclonal antibody specifically detects CD5 in FACS, IF, IHC (fro) and IHC (af). It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for CD5 antibody (ABIN965806)

Target: CD5

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD5 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Acetone-fixed) (IHC (af))

Clone: B390 (B-B8)

Product Details anti-CD5 Antibody

Specificity: This antibody reacts with a 67 kD molecule present on the surface of more than 95% normal human T-cells.

Purification: Purified.

Immunogen: Human T cells, activated with PHA for 3 days.

Isotype: IgG1

Application Details

Application Notes: Immunofluorescence: 5-20 ug/mL.
Flow cytometry: 1-5 ug/10^6 cells.
Immunohistochemistry: 5-20 ug/mL on frozen sections using avidin-biotin system. This antibody was not quality controlled in flow cytometry.
Staining Procedure: This antibody can be used on acetone fixed frozen cryostat sections.

Comment:

Recommended Positive Control: Tonsil, Lymphoid Tissue

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.4 mg/ml.

Buffer: 50 mM Sodium Borate, 150 mM Sodium Chloride, 20% Glycerol, pH 8.0

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-CD5 antibody (ABIN965806)

Ledbetter, Parsons, Martin, Hansen, Rabinovitch, June: "Antibody binding to CD5 (Tp67) and Tp44 T cell surface molecules: effects on cyclic nucleotides, cytoplasmic free calcium, and cAMP-mediated suppression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 137, Issue 10, pp. 3299-305, (1986) (PubMed).

Target Details for CD5

Target: CD5

Gene ID: 921