CD56 antibody

Catalog No. ABIN7446175

This Mouse Monoclonal antibody specifically detects CD56 in FACS and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for CD56 antibody (ABIN7446175)

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD56 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: B332(123A8)

Product Details anti-CD56 Antibody

Specificity: This antibody reacts with a 135/220 kD glycosylated and sialyated protein, expressed on CD2+, CD3-, CD16+ natural killer cells and neuroectodermal cells.

Purification: Purified.

Immunogen: Membrane preparation from small cell lung carcinoma.

Isotype: IgG1

Application Details

Application Notes: Immunofluorescence: 10-20 micrograms/mL.
Flow cytometry: 1-5 ug/10^6 cells.
Westernblotting: 1-5 ug/mL.
Immunohistochemistry: 4 -5 micrograms/mL on frozen and formalin fixed tissue sections using avidin-biotin system. The formalin fixed tissue requires boiling in 10 mM citrate buffer, pH 6.0 for 10-20 mins. This antibody is used for identification of NK- cells.
This product was not quality controlled in flow cytometry.
Staining Procedure: This antibody can be used on acetone fixed frozen cryostat sections. This antibody can be used with formalin-fixed paraffin-embedded tissue sections. These tissue require boiling in 10 mM citrate buffer, pH 6.0 for 10-20 mins. followed by cooling at RT. The antibody may be used at a dilution of 2-4 micrograms/mL in IHC.

Comment:

Recommended Positive Control: Human retinoblastoma or neuroblastoma, small Granulocytes Lung Carcinoma or wilms tumors.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/ml.

Buffer: 10 mM Sodium phosphate, 150 mM Sodium Chloride, 0.2% BSA, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for CD56

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Alternative Name: CD56,Natural Killer Cell

Molecular Weight: 125, 145, 180 kDa.

Gene ID: 4684