CD74, MHC CLASS11 INVARIANT CHAIN antibody

Catalog No. ABIN965816

The Mouse Monoclonal anti- antibody has been validated for IHC (fro), FACS, IF and WB. It is suitable to detect in samples from Human.

Quick Overview for CD74, MHC CLASS11 INVARIANT CHAIN antibody (ABIN965816)

Target: CD74, MHC Class 11 invariant Chain

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)

Clone: B318 (LN-2)

Product Details

Specificity: This antibody reacts with several isoforms of the CD74 antigen, the invariant chain of HLA-DR.

Purification: Purified.

Immunogen: Cell extracts from diffuse histolytic lymphoma SU-DHL-4 cells.

Isotype: IgG1

Application Details

Application Notes: Immunofluorescence: 10-20 ug/mL.
Flow cytometry: 1-5 ug/10^6 cells.
Immunoblotting: 1-5 ug/mL.
Immunohistochemistry: 10-20 ug/mL on frozen or formalin fixed paraffin embedded tissue sections using avidin-biotin system.
This product was not quality controlled in flow cytometry.
Staining Procedure: This antibody can be used on frozen cryostat sections as well as formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation is buffered formalin can destroy the epitope. The antibody may be used at a dilution of 10-20 ug/mL in IHC. The optimal conditions should be determined by the individual laboratory. When using paraffin-embedded and formalin-fixed tissues, high temperature antigenic unmasking technique (see instructions below) is strongly recommended for consistent and reproducible results This antibody can be used on frozen cryostat sections as well as formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation is buffered formalin can destroy the epitope. The antibody may be used at a dilution of 10-20 ug/mL in IHC. The optimal conditions should be determined by the individual laboratory. When using paraffin-embedded and formalin-fixed tissues, high temperature antigenic unmasking technique is strongly recommended for consistent and reproducible results

Comment:

Recommended Positive Control: Tonsil

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.4 mg/ml.

Buffer: 50 mM Sodium Borate, 150 mM Sodium Chloride, 20% Glycerol, pH 8.0

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details

Target: CD74, MHC Class 11 invariant Chain

Alternative Name: CD74,MHC Class11 Invariant Chain

Gene ID: 972