CD8 antibody

Catalog No. ABIN965818

Product Details anti-CD8 Antibody

Target: CD8

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Specificity: This antibody reacts with a 32 kD molecule present on human suppressor/cytotoxic T-cells. These antibodies also react with sinus-lining cells in the human spleen.

Purification: Purified.

Immunogen: E-rosette forming cells purified from human PBL.

Clone: B334 (H030-1-2)

Isotype: IgM

Application Details

Application Notes: Immunofluorescence: 5-20 ug/mL.
Flow cytometry: 1-5 ug/10^6 cells.
Immunohistochemistry: 5-20 ug/mL on frozen sections using avidin-biotin system. This antibody was not quality controlled in flow cytometry.
Staining Procedure: This antibody can be used on frozen cryostat sections as well as formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation is buffered formalin can destroy the epitope.

Comment:

Recommended Positive Control: Tonsil and Lymph Node

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.4 mg/ml.

Buffer: 50 mM Sodium Borate, 150 mM Sodium Chloride, 20% Glycerol, pH 8.0

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-CD8 antibody (ABIN965818)

Reinherz, Kung, Goldstein, Schlossman: "A monoclonal antibody reactive with the human cytotoxic/suppressor T cell subset previously defined by a heteroantiserum termed TH2." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 124, Issue 3, pp. 1301-7, (1980) (PubMed).

Target Details for CD8

Target: CD8

Abstract: CD8 Products

Gene ID: 925