CDW78, , BA ANTIGEN (Leu21) antibody

Catalog No. ABIN965851

This Mouse Monoclonal antibody specifically detects in IHC (fp), IHC (fro), FACS and IF. It exhibits reactivity toward Human.

Quick Overview for CDW78, , BA ANTIGEN (Leu21) antibody (ABIN965851)

Target: CDW78, , BA ANTIGEN

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: B351(DF1588)

Product Details

Binding Specificity: Leu21

Specificity: Cdw78, also known as Ba antigen, Leu21 or LO-panB-a, is present on some immature and some mature Bcells. Its expression is greatly increased after B-cell activation in vitro. It is also found on tissue macrophages and on epithelial cells but not on T-cells, NK cells, monocytes, granulocytes, or myeloid tissues.

Purification: Purified.

Isotype: IgG1 kappa

Application Details

Application Notes: Immunofluorescence: 5-20 ug/mL.
Flow cytometry: 1-5 ug/10^6 cells.
Immunoblotting: 1-5 ug/mL.
Immunohistochemistry: 5-20 ug/mL on frozen sections using avidin-biotin system.
This product was not quality controlled in flow cytometry.
Staining Procedure: This antibody can be used on frozen cryostat sections as well as formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation is buffered formalin can destroy the epitope. The antibody may be used at a dilution of 5-10 mg/mL in IHC.

Comment:

Recommended Positive Control: N/A

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.4 mg/ml.

Buffer: 50 mM Sodium Borate, 150 mM Sodium Chloride, 20% Glycerol, pH 8.0

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details

Target: CDW78, , BA ANTIGEN