CrkL antibody

Catalog No. ABIN965932

This Mouse Monoclonal antibody specifically detects CrkL in WB, ELISA and FACS. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for CrkL antibody (ABIN965932)

Target: CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CrkL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Clone: 5E12

Product Details anti-CrkL Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human CRKL expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes: Western Bloting: 1/500 - 1/2000.
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-CrkL antibody (ABIN965932)

Seo, Suenaga, Hatakeyama, Taiji, Imamoto: "Structural and functional basis of a role for CRKL in a fibroblast growth factor 8-induced feed-forward loop." in: Molecular and cellular biology, Vol. 29, Issue 11, pp. 3076-87, (2009) (PubMed).

Target Details for CrkL

Target: CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))

Alternative Name: CRKL

Background: This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.
Synonyms: CRKL

Molecular Weight: 39kDa

Gene ID: 1399

Pathways: Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Signaling of Hepatocyte Growth Factor Receptor