COX1 antibody (N-Term)

Catalog No. ABIN965953

This Rabbit Polyclonal antibody specifically detects COX1 in WB. It exhibits reactivity toward Human and has been mentioned in 4+ publications.

Quick Overview for COX1 antibody (N-Term) (ABIN965953)

Target: COX1 (Cytochrome C Oxidase Subunit I (COX1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COX1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-COX1 Antibody

Binding Specificity: N-Term

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Cytochrome c oxidase subunit 1 (MT-CO1) detection. Tested with WB in Human.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminal of human CO1.< br/>Immunogen was affinity purified.

Isotype: IgG

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: At -20 °C for one year. After reconstitution, at 4 °C for one month. It can also be aliquotted and stored frozen at -20 °C for a longer time.

References for anti-COX1 antibody (ABIN965953)

Acín-Pérez, Bayona-Bafaluy, Bueno, Machicado, Fernández-Silva, Pérez-Martos, Montoya, López-Pérez, Sancho, Enríquez: "An intragenic suppressor in the cytochrome c oxidase I gene of mouse mitochondrial DNA." in: Human molecular genetics, Vol. 12, Issue 3, pp. 329-39, (2003) (PubMed).

Yamada, Andrews, Chan, McKeown, Magli, de Berardinis, Loewenstein, Lazar, OKeefe, Letson, London, Ruttum, Matsumoto, Saito, Morris, Del Monte, Johnson, Uyama, Houtman, de Vries, Carlow, Hart, Krawiecki, Shoffner, Vogel, Katowitz, Goldstein, Levin, Sener, : "Heterozygous mutations of the kinesin KIF21A in congenital fibrosis of the extraocular muscles type 1 (CFEOM1)." in: Nature genetics, Vol. 35, Issue 4, pp. 318-21, (2003) (PubMed).

Hosler, Ferguson-Miller, Calhoun, Thomas, Hill, Lemieux, Ma, Georgiou, Fetter, Shapleigh: "Insight into the active-site structure and function of cytochrome oxidase by analysis of site-directed mutants of bacterial cytochrome aa3 and cytochrome bo." in: Journal of bioenergetics and biomembranes, Vol. 25, Issue 2, pp. 121-36, (1993) (PubMed).

Kadenbach, Jarausch, Hartmann, Merle: "Separation of mammalian cytochrome c oxidase into 13 polypeptides by a sodium dodecyl sulfate-gel electrophoretic procedure." in: Analytical biochemistry, Vol. 129, Issue 2, pp. 517-21, (1983) (PubMed).

Target Details for COX1

Target: COX1 (Cytochrome C Oxidase Subunit I (COX1))

Alternative Name: Cytochrome c oxidase subunit 1

Background: Cytochrome c oxidase subunit I (CO1 or MTCO1) is 1 of 3 mitochondrial DNA (mtDNA) encoded subunits (MTCO1, MTCO2, MTCO3) of respiratory Complex IV. Complex IV is located within the mitochondrial inner membrane and is the third and final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. It is composed of 13 polypeptides. Subunits I, II, and III (MTCO1, MTCO2, MTCO3) are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc, and VIII are nuclear encoded. The cytochrome c oxidase family of enzymes have 4 redox centers, 2 hemes and 2 copper centers. In mitochondrial Complex IV, the 2 hemes are a and a3 and the 2 coppers are CuA and CuB. The 2 hemes and CuB are bound to subunit I. Acin-Perez et al. (2003) identified a cell line containing single and double missense mutations in the cytochrome c oxidase (COX) subunit I gene of mouse mitochondrial DNA. And they hypothesized that deleterious mutations can arise and become predominant, cultured cells can maintain several mtDNA haplotypes at stable frequencies, the respiratory chain has little spare COX capacity, and that the size of a cavity in the vicinity of val421 in MTCO1I of animal COX may affect the function of the enzyme.

Pathways: Proton Transport