MAPK6 antibody

Catalog No. ABIN966092

The Mouse Monoclonal anti-MAPK6 antibody has been validated for WB, ELISA and FACS. It is suitable to detect MAPK6 in samples from Human. There are 2+ publications available.

Quick Overview for MAPK6 antibody (ABIN966092)

Target: MAPK6 (Mitogen-Activated Protein Kinase 6 (MAPK6))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAPK6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Clone: 4E8

Product Details anti-MAPK6 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human ERK3 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes: Western Bloting: 1/500 - 1/2000.
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-MAPK6 antibody (ABIN966092)

Klinger, Turgeon, Lévesque, Wood, Aagaard-Tillery, Meloche: "Loss of Erk3 function in mice leads to intrauterine growth restriction, pulmonary immaturity, and neonatal lethality." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 39, pp. 16710-5, (2009) (PubMed).

Déléris, Rousseau, Coulombe, Rodier, Tanguay, Meloche: "Activation loop phosphorylation of the atypical MAP kinases ERK3 and ERK4 is required for binding, activation and cytoplasmic relocalization of MK5." in: Journal of cellular physiology, Vol. 217, Issue 3, pp. 778-88, (2008) (PubMed).

Target Details for MAPK6

Target: MAPK6 (Mitogen-Activated Protein Kinase 6 (MAPK6))

Alternative Name: ERK3

Background: The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters.
Tissue specificity: Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts
Synonyms: ERK3, PRKM6, p97MAPK, HsT17250, DKFZp686F03189, MAPK6

Molecular Weight: 105kDa

Gene ID: 5596

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C