Filamin-Like antibody

Catalog No. ABIN966145

This Mouse Monoclonal antibody specifically detects Filamin-Like in IHC (fp), ELISA and WB. It exhibits reactivity toward Human, Chicken, Mouse and Rat.

Quick Overview for Filamin-Like antibody (ABIN966145)

Target: Filamin-Like (LOC409697)

Reactivity: Human, Chicken, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Application: Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), ELISA, Western Blotting (WB)

Clone: FLMN01 (same as PM6-317)

Product Details anti-Filamin-Like Antibody

Purification: Concentrated. Purified from the ascites fluid by Protein G chromatography

Immunogen: Platelet Filamin

Isotype: IgG1

Application Details

Application Notes: ELISA (Order Ab without BSA)
Western blotting: (Ab 1-2 micrograms/ml for 2hrs at RT)
Immunohistology (Formalin/paraffin)(Use Ab at 1-2 micrograms/ml for 30 min at RT)
Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

Comment:

Cellular Localization: Cytoplasmic.
Recommended Positive Control: NIH3T3 Cells, Skin

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL.

Buffer: 10mM PBS, pH 7.4, with 0.2% BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for Filamin-Like

Target: Filamin-Like (LOC409697)

Alternative Name: Filamin

Background: Filamin functions as a cross-linking protein forming a flexible link between two actin filaments. It is composed of two identical polypeptide chains each joined to the other at one end, with an actin binding site at the other. It is present in human platelets, lymphocytes, fibroblasts, and smooth muscle actin.
Also available without BSA and azide at 1mg/ml.

Molecular Weight: 250kDa