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GAPDH antibody

GAPDH Reactivity: Human WB, ELISA, IHC, IF Host: Mouse Monoclonal 1A10 unconjugated
Catalog No. ABIN966180
  • Target See all GAPDH Antibodies
    GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
    Reactivity
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    • 63
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    Human
    Host
    • 161
    • 83
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    Mouse
    Clonality
    • 184
    • 89
    Monoclonal
    Conjugate
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    This GAPDH antibody is un-conjugated
    Application
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    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)
    Purification
    Ascitic fluid
    Immunogen
    Purified recombinant fragment of human GAPDH expressed in E. Coli.
    Clone
    1A10
    Isotype
    IgG1
    Top Product
    Discover our top product GAPDH Primary Antibody
  • Application Notes
    Western Bloting: 1/500 - 1/2000.
    Immunohistochemistry: 1/200 - 1/1000.
    Immunofluorescence: 1/200 - 1/1000.
    ELISA: Propose dilution 1/10000.
    Not yet tested in other applications.
    Determining optimal working dilutions by titration test.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
  • Target
    GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
    Alternative Name
    GAPDH (GAPDH Products)
    Background
    Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. It catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age- related neuronal diseases, such as Alzheimer's and Huntington's disease.
    Synonyms: G3PD, GAPD, MGC88685
    Molecular Weight
    37kDa
    Gene ID
    2597
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