GST antibody

Catalog No. ABIN966238

This Monoclonal antibody specifically detects GST in WB and ELISA. It exhibits reactivity toward Schistosoma japonicumand has been mentioned in 1 publication.

Quick Overview for GST antibody (ABIN966238)

Target: GST (Glutathione S Transferase (GST))

Reactivity: Schistosoma japonicum

Host: Please inquire

Clonality: Monoclonal

Conjugate: This GST antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-GST Antibody

Specificity: Ni-NTA purified recombinant GST expressed in E. Coli strain M15.

Purification: Antibodies are purified by protein A affinity chromatography

Isotype: IgG1

Application Details

Application Notes: Western Blot: Dilution 1: 2000- 5,000
ELISA: Proposed dilution 1: 10,000.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Storage: -20 °C

References for anti-GST antibody (ABIN966238)

Smith, Johnson: "Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase." in: Gene, Vol. 67, Issue 1, pp. 31-40, (1988) (PubMed).

Target Details for GST

Target: GST (Glutathione S Transferase (GST))

Alternative Name: Glutathione-S-Transferase (GST)

Background: Plasmid vectors for the expression of coding regions of eukaryotic genes in E. coliare in common usage, such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. coli.