LIM Domain Kinase 1 antibody

Catalog No. ABIN966485

The Rabbit Polyclonal anti-LIM Domain Kinase 1 antibody has been validated for IHC. It is suitable to detect LIM Domain Kinase 1 in samples from Human, Mouse and Rat. There are 3+ publications available.

Quick Overview for LIM Domain Kinase 1 antibody (ABIN966485)

Target: LIM Domain Kinase 1 (LIMK1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIM Domain Kinase 1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-LIM Domain Kinase 1 Antibody

Specificity: LIMK1 (Ab-508) antibody detects endogenous levels of total LIMK1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human LIMK1 around the phosphorylation site of threonine 508 (R-Y-TP-V-V).

Application Details

Application Notes: WB: 1:500~1:1000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100ug/100ul.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-LIM Domain Kinase 1 antibody (ABIN966485)

Soosairajah, Maiti, Wiggan, Sarmiere, Moussi, Sarcevic, Sampath, Bamburg, Bernard: "Interplay between components of a novel LIM kinase-slingshot phosphatase complex regulates cofilin." in: The EMBO journal, Vol. 24, Issue 3, pp. 473-86, (2005) (PubMed).

Ohashi, Nagata, Maekawa, Ishizaki, Narumiya, Mizuno: "Rho-associated kinase ROCK activates LIM-kinase 1 by phosphorylation at threonine 508 within the activation loop." in: The Journal of biological chemistry, Vol. 275, Issue 5, pp. 3577-82, (2000) (PubMed).

Edwards, Sanders, Bokoch, Gill: "Activation of LIM-kinase by Pak1 couples Rac/Cdc42 GTPase signalling to actin cytoskeletal dynamics." in: Nature cell biology, Vol. 1, Issue 5, pp. 253-9, (1999) (PubMed).

Target Details for LIM Domain Kinase 1

Target: LIM Domain Kinase 1 (LIMK1)

Alternative Name: LIMK1

Pathways: Caspase Cascade in Apoptosis, Regulation of Cell Size, CXCR4-mediated Signaling Events