DOK1 antibody

Catalog No. ABIN966767

The Rabbit Polyclonal anti-DOK1 antibody has been validated for IHC. It is suitable to detect DOK1 in samples from Human, Mouse and Rat. There are 4+ publications available.

Quick Overview for DOK1 antibody (ABIN966767)

Target: DOK1 (Docking Protein 1, 62kDa (Downstream of tyrosine Kinase 1) (DOK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DOK1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-DOK1 Antibody

Specificity: P62Dok (Ab-398) antibody detects endogenous levels of total p62Dok protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human p62Dok around the phosphorylation site of tyrosine 398 (E-G-YP-E-L).

Application Details

Application Notes: WB: 1:500~1:1000
IHC: 1:50~1:100.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100ug/100ul.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-DOK1 antibody (ABIN966767)

Simoncic, Bourdeau, Lee-Loy, Rohrschneider, Tremblay, Stanley, McGlade: "T-cell protein tyrosine phosphatase (Tcptp) is a negative regulator of colony-stimulating factor 1 signaling and macrophage differentiation." in: Molecular and cellular biology, Vol. 26, Issue 11, pp. 4149-60, (2006) (PubMed).

Yopp, Ochando, Mao, Ledgerwood, Ding, Bromberg: "Sphingosine 1-phosphate receptors regulate chemokine-driven transendothelial migration of lymph node but not splenic T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 5, pp. 2913-24, (2005) (PubMed).

Dubé, Cheng, Tremblay: "The role of protein tyrosine phosphatase 1B in Ras signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 7, pp. 1834-9, (2004) (PubMed).

Wick, Dong, Hu, Langlais, Liu: "Insulin receptor-mediated p62dok tyrosine phosphorylation at residues 362 and 398 plays distinct roles for binding GTPase-activating protein and Nck and is essential for inhibiting insulin-stimulated activation of Ras and Akt." in: The Journal of biological chemistry, Vol. 276, Issue 46, pp. 42843-50, (2001) (PubMed).

Target Details for DOK1

Target: DOK1 (Docking Protein 1, 62kDa (Downstream of tyrosine Kinase 1) (DOK1))

Alternative Name: p62Dok