CD90 antibody

Catalog No. ABIN967178

The Rat Monoclonal anti-CD90 antibody has been validated for IHC (fro). It is suitable to detect CD90 in samples from Mouse. There is 1 publication available.

Quick Overview for CD90 antibody (ABIN967178)

Target: CD90 (THY1)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD90 antibody is un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CD90 Antibody

Specificity: This antibody reacts with mouse Thy-1, a well characterized cell surface glycoprotein with a broad tissue distribution. Thy-1 is present on mouse brain, thymocytes, peripheral T-lymphocytes and some intraepithelial Tlymphocytes. This antigen is likely to play a role in the development and function of the nervous system.

Purification: Concentrated.

Immunogen: EL-4 mouse T cell lymphoma cells.

Isotype: IgG2b kappa

Application Details

Application Notes: Immunohistochemistry: (Aceone-fixed frozen) 1:50-1:100
Flow: 1:100-1:300 (Not tested in our lab.)
IF -1:50-1:200
Staining Procedure: This antibody can be used on frozen tissue sections only. The antibody may be used at a dilution 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.

Comment:

Cellular Localization: Cell membrane.
Recommended Positive Control: Mouse thymus

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.3 mg/ml.

Buffer: 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-CD90 antibody (ABIN967178)

Beissert, He, Hueber, Lellouch, Metze, Mehling, Luger, Schwarz, Grabbe: "Impaired cutaneous immune responses in Thy-1-deficient mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 10, pp. 5296-302, (1998) (PubMed).

Target Details for CD90

Target: CD90 (THY1)

Alternative Name: Thy-1

Molecular Weight: 25 kDa

Gene ID: 7070

Pathways: Cell-Cell Junction Organization