Thyroglobulin antibody

Catalog No. ABIN967179

Product Details anti-Thyroglobulin Antibody

Target: Thyroglobulin (TG)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Thyroglobulin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purification: Concentrated.

Immunogen: Purified Human Thyroglobulin

Clone: B34-1

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistochemistry: (Paraffin/Frozen): 1:50-1:100.
Staining Procedure: Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 to 1:100 with AutoProbe III (The optimal conditions should be determined by the individual laboratory.

Comment:

Cellular Localization: Cytoplasmic.
Recommended Positive Control: Thyroid Tissue

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/ml

Buffer: 20 mM Tris-Borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing Fetal Bovine Serum, BMC-6 Carrier Polysaccharides, Carrier Protein, pH 7.5

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-Thyroglobulin antibody (ABIN967179)

Ossendorp, Leer, Bruning, van den Brink, Sterk, de Vijlder: "Iodination of newly synthesized thyroglobulin by FRTL-5 cells is selective and thyrotropin dependent." in: Molecular and cellular endocrinology, Vol. 66, Issue 2, pp. 199-205, (1990) (PubMed).

Ossendorp, Bruning, Schuuring, Van Den Brink, van der Heide, De Vijlder, De Bruin: "Thyrotropin dependent and independent thyroid cell lines selected from FRTL-5 derived tumors grown in nude mice." in: Endocrinology, Vol. 127, Issue 1, pp. 419-30, (1990) (PubMed).

Ossendorp, Bruning, Van den Brink, De Boer: "Efficient selection of high-affinity B cell hybridomas using antigen-coated magnetic beads." in: Journal of immunological methods, Vol. 120, Issue 2, pp. 191-200, (1989) (PubMed).

Target Details for Thyroglobulin

Target: Thyroglobulin (TG)

Alternative Name: Thyroglobulin (TG Products)

Background: The antibody to thyroglobulin has been shown to be useful in positive identification of thyroid carcinomas of the papillary and follicular types. Adenocarcinomas of non-thyroidal origin are not reactive.

Gene ID: 7038

Pathways: Thyroid Hormone Synthesis