CD43 antibody

Catalog No. ABIN967347

Product Details anti-CD43 Antibody

Target: CD43 (SPN) (Sialophorin (SPN))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD43 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Functional Studies (Func)

Brand: BD Pharmingen™

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: L60

Isotype: IgG1 kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing no preservative.

Preservative: Without preservative

Storage: 4 °C

Storage Comment: This preparation contains no preservatives, thus it should be handled under aseptic conditions. Store undiluted at 4° C.

References for anti-CD43 antibody (ABIN967347)

Bazil, Strominger: "CD43, the major sialoglycoprotein of human leukocytes, is proteolytically cleaved from the surface of stimulated lymphocytes and granulocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 9, pp. 3792-6, (1993) (PubMed).

Ardman, Sikorski, Staunton: "CD43 interferes with T-lymphocyte adhesion." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 11, pp. 5001-5, (1992) (PubMed).

Campanero, Pulido, Alonso, Pivel, Pimentel-Muiños, Fresno, Sánchez-Madrid: "Down-regulation by tumor necrosis factor-alpha of neutrophil cell surface expression of the sialophorin CD43 and the hyaluronate receptor CD44 through a proteolytic mechanism." in: European journal of immunology, Vol. 21, Issue 12, pp. 3045-8, (1992) (PubMed).

Cyster, Williams: "The importance of cross-linking in the homotypic aggregation of lymphocytes induced by anti-leukosialin (CD43) antibodies." in: European journal of immunology, Vol. 22, Issue 10, pp. 2565-72, (1992) (PubMed).

Kuijpers, Hoogerwerf, Kuijpers, Schwartz, Harlan: "Cross-linking of sialophorin (CD43) induces neutrophil aggregation in a CD18-dependent and a CD18-independent way." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 3, pp. 998-1003, (1992) (PubMed).

Rieu, Porteu, Bessou, Lesavre, Halbwachs-Mecarelli: "Human neutrophils release their major membrane sialoprotein, leukosialin (CD43), during cell activation." in: European journal of immunology, Vol. 22, Issue 11, pp. 3021-6, (1992) (PubMed).

Rosenstein, Park, Hahn, Rosen, Bierer, Burakoff: "CD43, a molecule defective in Wiskott-Aldrich syndrome, binds ICAM-1." in: Nature, Vol. 354, Issue 6350, pp. 233-5, (1992) (PubMed).

Segal, Stoler, Tubbs: "The "CD43 only" phenotype. An aberrant, nonspecific immunophenotype requiring comprehensive analysis for lineage resolution." in: American journal of clinical pathology, Vol. 97, Issue 6, pp. 861-5, (1992) (PubMed).

Park, Rosenstein, Remold-ODonnell, Bierer, Rosen, Burakoff: "Enhancement of T-cell activation by the CD43 molecule whose expression is defective in Wiskott-Aldrich syndrome." in: Nature, Vol. 350, Issue 6320, pp. 706-9, (1991) (PubMed).

Target Details for CD43

Target: CD43 (SPN) (Sialophorin (SPN))

Alternative Name: CD43 (Leukosialin) (SPN Products)

Background: Anti-leukosialin monoclonal antibody (clone L60) recognizes a sialic acid-dependent determinant on leukosialin, a strongly anionic, highly o-linked glycosylated cell surface protein with a 45 nm extracellular domain. Leukosialin, also know as sialophorin or CD43, is a major component of T lymphocyte, thymocyte, and neutrophil (PMN) membranes, and is also expressed on many different cells including monocytes, platelets, bone marrow stem cells, and plasma cells.

Pathways: Regulation of Leukocyte Mediated Immunity