MHC, Class I antibody

Catalog No. ABIN967374

This anti-MHC, Class I antibody is a Mouse Monoclonal antibody detecting MHC, Class I in FACS, WB, IP, ELISA and IHC (f). Suitable for Mouse. This Primary Antibody has been cited in 3+ publications.

Quick Overview for MHC, Class I antibody (ABIN967374)

Target: MHC, Class I

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This MHC, Class I antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), ELISA, Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: SF1-1-1

Product Details anti-MHC, Class I Antibody

Brand: BD Pharmingen™

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE™ (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: BALB/c mouse cells

Isotype: IgG2a kappa

Application Details

Application Notes: For immunohistochemical staining (IHC) of acetone-fixed frozen sections, we recommend the use of biotinylated SF1-1.1 mAb.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4° C.

References for anti-MHC, Class I antibody (ABIN967374)

Noun, Reboul, Abastado, Jaulin, Kourilsky, Pla: "Alloreactive monoclonal antibodies select Kd molecules with different peptide profiles." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 6, pp. 2455-61, (1996) (PubMed).

Zhao, Iwata: "Cross-linking of the TCR-CD3 complex with CD4, CD8 or LFA-1 induces an anti-apoptotic signal in thymocytes: the signal is canceled by FK506." in: International immunology, Vol. 7, Issue 9, pp. 1387-96, (1996) (PubMed).

Abastado, Casrouge, Kourilsky: "Differential role of conserved and polymorphic residues of the binding groove of MHC class I molecules in the selection of peptides." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 7, pp. 3569-75, (1993) (PubMed).

Target Details for MHC, Class I

Target: MHC, Class I

Alternative Name: H-2K d

Background: The SF1-1.1 antibody reacts with the alpha3 domain of the H-2K[d] MHC class I alloantigen. Reactivity with other haplotypes (e.g, b, j, k, p, q, s, v) has not been observed. It has been reported that plate-bound SF1-1.1 mAb moderately enhances the apoptotic response of thymocytes to plate-bound 145-2C11 mAb (anti-mouse CD3e, ABIN967568 and ABIN967370). This antibody is routinely tested by flow cytometric analysis.