SV40 Large T Antigen antibody

Catalog No. ABIN967411

This Mouse Monoclonal antibody specifically detects SV40 Large T Antigen in IP, WB, IF and IHC (f). It exhibits reactivity toward Virus. It has been mentioned in 3+ publications

Quick Overview for SV40 Large T Antigen antibody (ABIN967411)

Target: SV40 Large T Antigen

Reactivity: Virus

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunoprecipitation (IP), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: PAb 101

Product Details

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: SV40-transformed BALB/c mouse cell lines

Isotype: IgG2a

Application Details

Application Notes: T-ag purified with PAb 101 has been shown to retain helicase activity. T-ag is immunoprecipitated as a single or multiple bands between about 80-95 kD depending on post-translational modifications. SV40-transformed cells such as COS-7 (ATCC CRL 1651) are suggested as positive controls. Any cell line that is not SV40-transformed or SV40-infected can be used as a negative control.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References

Gurney, Tamowski, Deppert: "Antigenic binding sites of monoclonal antibodies specific for simian virus 40 large T antigen." in: Journal of virology, Vol. 57, Issue 3, pp. 1168-72, (1986) (PubMed).

Scheller, Covey, Barnet, Prives: "A small subclass of SV40 T antigen binds to the viral origin of replication." in: Cell, Vol. 29, Issue 2, pp. 375-83, (1982) (PubMed).

Gurney, Harrison, Fenno: "Monoclonal antibodies against simian virus 40 T antigens: evidence for distinct sublcasses of large T antigen and for similarities among nonviral T antigens." in: Journal of virology, Vol. 34, Issue 3, pp. 752-63, (1980) (PubMed).

Target Details

Target: SV40 Large T Antigen

Alternative Name: SV40 Large T

Target Type: Viral Protein

Background: Simian virus 40 is a small DNA virus encoded by 5.2 kb of double-stranded DNA. SV40 large T antigen (T-ag) is a multifunctional ~85 kD phosphoprotein, which is the sole viral protein required for SV40 replication. All other factors are provided by the infected host cell. In addition to its role in SV40 DNA replication, T-ag also causes transformation of susceptible cell lines. Studies of various mutant T-ag proteins have shown that the replication and transformation fractions of T-ag can be separated. The multifunctional nature of this protein has resulted in its use as a model system in a wide variety of disciplines. SV40 T-ag exercises negative regulation on the transcription of SV40 early mRNA by feedback inhibition and exerts positive regulation on transcription from the late promoter. In addition to transcriptional regulation, T-ag is involved in viral DNA replication. Specific biochemical functions required for DNA synthesis that are inherent to the T-ag include high-affinity binding to sites within the viral origin of DNA synthesis, and ATPase and helicase activities. Other functions attributed to T-ag include cellular transformation, induction of cellular DNA synthesis, induction of rRNA synthesis and provision of a host-range function for viral replication. However, all functions of T-ag are influenced by a wide range of post-translational modifications including phosphorylation, glycosylation, acetylation, acylation and adenylation. T-ag exists in monomeric as well as polymeric forms and associates with the tumor suppressor proteins p53 and Rb (retinoblastoma protein). Most of T-ag is transported to the nucleus, while a small fraction is localized at the cell surface. PAb 101 recognizes a C-terminal epitope within the last 190 amino acids of T-ag. PAb 101 was originally known as Clone 7. Studies have suggested that PAb 101 binds the strongest to mature T-ag. PAb 101 (i.e., Clone 7) was developed along with a panel of monoclonal antibodies where SV40-transformed BALB/c mouse cell lines (SVT2 or B4) were used as immunogens. The specificity of the antibody was originally characterized by a variety of techniques using SV40-infected and SV40-transformed cells.