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Interferon gamma antibody

Name: Interferon gamma antibody
SKU: ABIN967461
Availability: OutOfStock

IFNG Reactivity: Mouse WB, ELISA, Neut, BR, Inhibition Host: Rat Monoclonal XMG1-2 unconjugated

See first image description (Interferon gamma antibody)

FACS

Expression of IFN-gamma by stimulated CD8+ and CD8-BALB/c spleen cells. (Interferon gamma antibody)

2 images

(2 references)

Catalog No. ABIN967461

Datasheet as PDF

Target See all Interferon gamma (IFNG) Antibodies

Interferon gamma (IFNG)
Reactivity
220

75

50

28

24

21

17

17

16

16

16

12

12

11

8

6

6

5

5

4

4

4

4

3

2

2

2

2

2

2

2

1
Mouse
Host
218

172

39

11

8

2

2

2
Rat
Compatible Secondaries
Clonality
260

193

1
Monoclonal
Conjugate
209

87

48

20

18

14

7

5

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
This Interferon gamma antibody is un-conjugated
Application
237

208

116

97

59

44

40

33

33

30

27

25

23

22

20

15

14

11

7

6

6

5

5

4

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1
Western Blotting (WB), ELISA, Neutralization (Neut), Blocking Reagent (BR), Blocking Antibody (Inhibition)

Brand

BD Pharmingen™

Characteristics

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen

Mouse IFN-gamma

Clone

XMG1-2

Isotype

IgG1 kappa

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IFNG Reactivity: Rat WB, IHC, IP, ICC Host: Mouse Monoclonal C8 unconjugated

Restrictions

For Research Use only
Format

Liquid

Concentration

0.5 mg/mL

Buffer

Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C

Storage Comment

Store undiluted at 4°C.
Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, (1992) (PubMed).
Target

Interferon gamma (IFNG)

Alternative Name

IFN-gamma (IFNG Products)

Background

The XMG1.2 antibody reacts with mouse interferon-gamma (IFN-gamma) protein. IFN-gamma is a pleiotropic cytokine, of approximately 15-17 kDa, involved in the regulation of the immune response. It plays an important role in activation, growth, and differentiation of T and B lymphocytes, macrophages, NK cells and other non-hematopoietic cell types. IFN-gamma production is associated with the Th-1 differentiation. This is a neutralizing antibody

Pathways

Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy