CD74 antibody

Catalog No. ABIN967482

This anti-CD74 antibody is a Rat Monoclonal antibody detecting CD74 in WB, IF, IP, IHC (f), IHC (fro), ICS and IHC (zinc). Suitable for Mouse. This Primary Antibody has been cited in 11+ publications.

Quick Overview for CD74 antibody (ABIN967482)

Target: CD74 (HLA-DR-gamma (CD74))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD74 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Intracellular Staining (ICS), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))

Clone: In-1

Product Details anti-CD74 Antibody

Brand: BD Pharmingen™

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: A.TL mouse lymphocytes and B10.A mouse-derived CH1 B-cell lymphoma

Isotype: IgG2b kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD74 antibody (ABIN967482)

Nakagawa, Roth, Wong, Nelson, Farr, Deussing, Villadangos, Ploegh, Peters, Rudensky: "Cathepsin L: critical role in Ii degradation and CD4 T cell selection in the thymus." in: Science (New York, N.Y.), Vol. 280, Issue 5362, pp. 450-3, (1998) (PubMed).

Bertolino, Rabourdin-Combe: "The MHC class II-associated invariant chain: a molecule with multiple roles in MHC class II biosynthesis and antigen presentation to CD4+ T cells." in: Critical reviews in immunology, Vol. 16, Issue 4, pp. 359-79, (1997) (PubMed).

Shachar, Flavell: "Requirement for invariant chain in B cell maturation and function." in: Science (New York, N.Y.), Vol. 274, Issue 5284, pp. 106-8, (1996) (PubMed).

Bodmer, Viville, Benoist, Mathis: "Diversity of endogenous epitopes bound to MHC class II molecules limited by invariant chain." in: Science (New York, N.Y.), Vol. 263, Issue 5151, pp. 1284-6, (1994) (PubMed).

Cresswell: "Assembly, transport, and function of MHC class II molecules." in: Annual review of immunology, Vol. 12, pp. 259-93, (1994) (PubMed).

Elliott, Drake, Amigorena, Elsemore, Webster, Mellman, Flavell: "The invariant chain is required for intracellular transport and function of major histocompatibility complex class II molecules." in: The Journal of experimental medicine, Vol. 179, Issue 2, pp. 681-94, (1994) (PubMed).

Bikoff, Huang, Episkopou, van Meerwijk, Germain, Robertson: "Defective major histocompatibility complex class II assembly, transport, peptide acquisition, and CD4+ T cell selection in mice lacking invariant chain expression." in: The Journal of experimental medicine, Vol. 177, Issue 6, pp. 1699-712, (1993) (PubMed).

Naujokas, Morin, Anderson, Peterson, Miller: "The chondroitin sulfate form of invariant chain can enhance stimulation of T cell responses through interaction with CD44." in: Cell, Vol. 74, Issue 2, pp. 257-68, (1993) (PubMed).

Viville, Neefjes, Lotteau, Dierich, Lemeur, Ploegh, Benoist, Mathis: "Mice lacking the MHC class II-associated invariant chain." in: Cell, Vol. 72, Issue 4, pp. 635-48, (1993) (PubMed).

Mehringer, Harris, Kindle, McCourt, Cullen: "Characterization of fragments of the murine Ia-associated invariant chain." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 3, pp. 920-7, (1991) (PubMed).

Koch, Koch, Hämmerling: "Ia invariant chain detected on lymphocyte surfaces by monoclonal antibody." in: Nature, Vol. 299, Issue 5884, pp. 644-5, (1982) (PubMed).

Target Details for CD74

Target: CD74 (HLA-DR-gamma (CD74))

Alternative Name: CD74

Background: The In-1 antibody reacts with CD74, the MHC class II-associated Invariant chain (Ii), expressed in I-A+ cell populations. Although it is predominantly expressed intracellularly, it has also been detected on the cell surface of some B-cell lines and at low levels on a small number of B lymphocytes. In the mouse, alternatively spliced transcripts encode 31-kDa and 41-kDa isoforms, and the cell-surface CD74 has a chondroitin sulfate side chain which allows it to interact with CD44. CD74 is directly involved in the intracellular transport of MHC class II molecules and antigen presentation, and it affects the maturation of T and B lymphocytes.
Synonyms: Ii

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints