CD9 antibody

Catalog No. ABIN967484

The Mouse Monoclonal anti-CD9 antibody has been validated for FACS, WB, IHC (p) and IHC (fro). It is suitable to detect CD9 in samples from Human. There are 2+ publications available.

Quick Overview for CD9 antibody (ABIN967484)

Target: CD9

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD9 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: M-L13

Product Details anti-CD9 Antibody

Brand: BD Pharmingen™

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotype: IgG1 kappa

Application Details

Application Notes: This reagent is effective for indirect immunofluorescence staining of human cells for flow cytometric analysis. It also worked by western blotting with the human platelets lysate at the concentration of 2-4 µg/ml (in-house data). It also can be used for immunohistochemistry on frozen tissue (spleen/thymus) sections at 2-10 µg/ml (in-house data). However, we do not recommend using this antibody on the formalin fixed paraffin tissue sections.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4° C.

References for anti-CD9 antibody (ABIN967484)

Masellis-Smith, Shaw: "CD9-regulated adhesion. Anti-CD9 monoclonal antibody induce pre-B cell adhesion to bone marrow fibroblasts through de novo recognition of fibronectin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 6, pp. 2768-77, (1994) (PubMed).

Cramer, Berger, Berndt: "Platelet alpha-granule and plasma membrane share two new components: CD9 and PECAM-1." in: Blood, Vol. 84, Issue 6, pp. 1722-30, (1994) (PubMed).

Target Details for CD9

Target: CD9

Alternative Name: CD9

Background: Reacts with a 24 kDa type III transmembrane protein which is expressed on platelets, pre-B cells, monocytes, endothelia, and epithelia. CD9 belongs to a family of membrane proteins called tetraspanins which transverse the membrane four times. CD9 is weakly expressed on resting mature B cells. M-L13 induces platelet aggregation and activation. This antibody is also suitable for staining acetone-fixed, frozen tissue sections.

Pathways: Response to Water Deprivation, Cell-Cell Junction Organization