CD35 antibody

Catalog No. ABIN967625

Product Details anti-CD35 Antibody

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD35 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Blocking Reagent (BR)

Brand: BD Pharmingen™

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Purified mouse CR1

Clone: 8C12

Isotype: IgG2a kappa

Application Details

Comment:

Related Products: ABIN967384

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4° C.

References for anti-CD35 antibody (ABIN967625)

Fischer, Goerg, Shen, Prodeus, Goodnow, Kelsoe, Carroll: "Dependence of germinal center B cells on expression of CD21/CD35 for survival." in: Science (New York, N.Y.), Vol. 280, Issue 5363, pp. 582-5, (1998) (PubMed).

Hu, Martin, Weis, Weis: "Expression of the murine CD21 gene is regulated by promoter and intronic sequences." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 10, pp. 4758-68, (1997) (PubMed).

Molina, Holers, Li, Fung, Mariathasan, Goellner, Strauss-Schoenberger, Karr, Chaplin: "Markedly impaired humoral immune response in mice deficient in complement receptors 1 and 2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 8, pp. 3357-61, (1996) (PubMed).

Ahearn, Fischer, Croix, Goerg, Ma, Xia, Zhou, Howard, Rothstein, Carroll: "Disruption of the Cr2 locus results in a reduction in B-1a cells and in an impaired B cell response to T-dependent antigen." in: Immunity, Vol. 4, Issue 3, pp. 251-62, (1996) (PubMed).

Martin, Weis: "Murine macrophages lack expression of the Cr2-145 (CR2) and Cr2-190 (CR1) gene products." in: European journal of immunology, Vol. 23, Issue 11, pp. 3037-42, (1993) (PubMed).

Yoshida, van den Berg, Dijkstra: "Two functionally different follicular dendritic cells in secondary lymphoid follicles of mouse spleen, as revealed by CR1/2 and FcR gamma II-mediated immune-complex trapping." in: Immunology, Vol. 80, Issue 1, pp. 34-9, (1993) (PubMed).

Kinoshita, Thyphronitis, Tsokos, Finkelman, Hong, Sakai, Inoue: "Characterization of murine complement receptor type 2 and its immunological cross-reactivity with type 1 receptor." in: International immunology, Vol. 2, Issue 7, pp. 651-9, (1991) (PubMed).

Heyman, Wiersma, Kinoshita: "In vivo inhibition of the antibody response by a complement receptor-specific monoclonal antibody." in: The Journal of experimental medicine, Vol. 172, Issue 2, pp. 665-8, (1990) (PubMed).

Kurtz, OToole, Christensen, Weis: "The murine complement receptor gene family. IV. Alternative splicing of Cr2 gene transcripts predicts two distinct gene products that share homologous domains with both human CR2 and CR1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 9, pp. 3581-91, (1990) (PubMed).

Target Details for CD35

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Alternative Name: CD35 (CR1) (CR1 Products)

Background: The 8C12 antibody recognizes an epitope present on the 190-kDa complement receptor protein, originally designated CR1 (CD35), but not the 145-150-kDa CR2 (CD21) molecule. Unlike the human system, in which these proteins are products of independent genes, both of these mouse receptors are membrane proteins resulting from the alternative splicing of mRNA transcribed from the Cr2 gene. Therefore, an alternative nomenclature has been proposed, designating the proteins Cr2-190 (CD21b) and Cr2-145 (CD21a), respectively. The epitope recognized by 8C12 mAb is only present on CD35/CD21b. Moreover, it has also been proposed that Crry is the true mouse genetic homologue of human CR1 (CD35). In the mouse, CD35 is expressed on the majority of peripheral B cells, on the majority of resident peritoneal macrophages, on peripheral blood granulocytes after treatment with N-formyl-Met-Leu-Phe, and on follicular dendritic cells, but not on thymocytes, T cells, erythrocytes, or platelets. In addition, it has not been detected, at the protein or mRNA level, in the macrophage cell line J774, bone marrow-derived macrophages, or thioglycollate-elicited peritoneal macrophages. The 8C12 mAb has been reported to inhibit rosette formation by C3bbearing sheep erythrocytes, to block the complement-dependent trapping of immune complexes by follicular dendritic cells, and to down-regulate mouse CD35 expression upon in vivo application, inhibiting only some primary antibody responses to immunization. B lymphocytes of Cr2[null] mice display impaired humoral immune responses in vivo. The 8C12 mAb recognizes an epitope on mouse CD35 distinct from the epitope recognized by anti-mouse CD21/CD35 mAb 7G6 (ABIN967384), and it does not block binding by 7G6 mAb to CD35. *Please note that the isotype of 8C12 mAb was originally reported to be Rat IgG2c. Further investigations have demonstrated that the isotype of 8C12 mAb is Rat IgG2a.
Synonyms: CR1, CD21b

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity