L-Selectin antibody

Catalog No. ABIN967639

Product Details anti-L-Selectin Antibody

Target: L-Selectin (SELL) (Selectin L (SELL))

Reactivity: Chemical

Host: Mouse

Clonality: Monoclonal

Conjugate: This L-Selectin antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Functional Studies (Func)

Brand: BD Pharmingen™

Characteristics: 1. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Peripheral Blood T Lymphocytes

Clone: SK11

Isotype: IgG2a kappa

Application Details

Application Notes: Functional Studies:
Anti-LECAM-1 monoclonal antibodies have been shown to inhibit neutrophil localization at sites of acute inflammation. Anti-LECAM-1 monoclonal antibody (clone SK11) also induces suppressor function in CD4+ T lymphocytes but does not induce lymphocyte proliferation.

Immunoblotting:
Using anti-LECAM-1 monoclonal antibody (clone SK11) in Western blot analysis of extracts from Leu- 8-transfected COS cells and the human T-cell leukemia line (Jurkat) revealed at least seven species of similar molecular weight and at least two closely spaced bands of 76 kD, the largest molecular weight form seen in COS cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4° C.

References for anti-L-Selectin antibody (ABIN967639)

Bevilacqua, Butcher, Furie, Furie, Gallatin, Gimbrone, Harlan, Kishimoto, Lasky, McEver: "Selectins: a family of adhesion receptors." in: Cell, Vol. 67, Issue 2, pp. 233, (1991) (PubMed).

Lawrence, Springer: "Leukocytes roll on a selectin at physiologic flow rates: distinction from and prerequisite for adhesion through integrins." in: Cell, Vol. 65, Issue 5, pp. 859-73, (1991) (PubMed).

Picker, Warnock, Burns, Doerschuk, Berg, Butcher: "The neutrophil selectin LECAM-1 presents carbohydrate ligands to the vascular selectins ELAM-1 and GMP-140." in: Cell, Vol. 66, Issue 5, pp. 921-33, (1991) (PubMed).

Polley, Phillips, Wayner, Nudelman, Singhal, Hakomori, Paulson: "CD62 and endothelial cell-leukocyte adhesion molecule 1 (ELAM-1) recognize the same carbohydrate ligand, sialyl-Lewis x." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 14, pp. 6224-8, (1991) (PubMed).

Smith, Kishimoto, Abbassi, Hughes, Rothlein, McIntire, Butcher, Anderson, Abbass: "Chemotactic factors regulate lectin adhesion molecule 1 (LECAM-1)-dependent neutrophil adhesion to cytokine-stimulated endothelial cells in vitro." in: The Journal of clinical investigation, Vol. 87, Issue 2, pp. 609-18, (1991) (PubMed).

Hallmann, Jutila, Smith, Anderson, Kishimoto, Butcher: "The peripheral lymph node homing receptor, LECAM-1, is involved in CD18-independent adhesion of human neutrophils to endothelium." in: Biochemical and biophysical research communications, Vol. 174, Issue 1, pp. 236-43, (1991) (PubMed).

Jutila, Rott, Berg, Butcher: "Function and regulation of the neutrophil MEL-14 antigen in vivo: comparison with LFA-1 and MAC-1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 143, Issue 10, pp. 3318-24, (1989) (PubMed).

Camerini, James, Stamenkovic, Seed: "Leu-8/TQ1 is the human equivalent of the Mel-14 lymph node homing receptor." in: Nature, Vol. 342, Issue 6245, pp. 78-82, (1989) (PubMed).

Kanof, Strober, James: "Induction of CD4 suppressor T cells with anti-Leu-8 antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 139, Issue 1, pp. 49-54, (1987) (PubMed).

Lewinsohn, Bargatze, Butcher: "Leukocyte-endothelial cell recognition: evidence of a common molecular mechanism shared by neutrophils, lymphocytes, and other leukocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 12, pp. 4313-21, (1987) (PubMed).

Michie, Garcia, Strickler, Dailey, Rouse, Warnke: "Expression of the Leu-8 antigen by B-cell lymphomas." in: American journal of clinical pathology, Vol. 88, Issue 4, pp. 486-90, (1987) (PubMed).

Target Details for L-Selectin

Target: L-Selectin (SELL) (Selectin L (SELL))

Alternative Name: CD62L (L-selectin) (SELL Products)

Target Type: Chemical

Background: Anti-LECAM-1 (Leu-8/TQ1) monoclonal antibody (clone SK11) recognizes an 80 kD protein on the surface of human leukocytes. The leukocyte-endothelial cell adhesion molecule 1 (LECAM-1) protein is the human homologue of the murine lymph node homing receptor, MEL-14, a molecule that initiates the adhesion of circulating leukocytes to high endothelium in postcapillary venules. LECAM-1 belongs to the selectin family of cell adhesion molecules and is also known as CD62L. This antibody is routinely tested by flow cytometric analysis.
Synonyms: L-Selectin