IFNGR1 antibody

Catalog No. ABIN967652

The Armenian Hamster Monoclonal anti-IFNGR1 antibody has been validated for FACS and IP. It is suitable to detect IFNGR1 in samples from Mouse. There is 1 publication available.

Quick Overview for IFNGR1 antibody (ABIN967652)

Target: IFNGR1 (Interferon gamma Receptor 1 (IFNGR1))

Reactivity: Mouse

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This IFNGR1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: 2E2

Product Details anti-IFNGR1 Antibody

Brand: BD Pharmingen™

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Purified preparation of soluble recombinant mouse IFN-gammaRalpha chain protein

Isotype: IgG1 kappa

Application Details

Application Notes: Immunofluorescent Staining and Flow Cytometric Analysis: The purified form of 2E2 can be used for the immunofluorescent staining (Less or equal than 1 µg antibody/10e6 cells) and flow cytometric analysis of normal mouse cells or cell lines to measure their expressed levels of IFN-gammaRalpha. An appropriate purified immunoglobulin isotype control is A19-3. A three-layer staining protocol is recommended for maximizing the detection IFN-gammaRalpha chains expressed by cells as detailed in the figure legend.
2E2 is a nonblocking antibody that can be used for the unobstructed immunofluorescent staining and flow cytometric analysis of cells in systems where the ligand (i.e., IFN-gamma) for IFN-gamma receptors is present. Immunoprecipitation: The 2E2 antibody has been reported to be useful for the immunoprecipitation of IFN-gammaRalpha chains from lysates of cloned mouse T cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4° C.

References for anti-IFNGR1 antibody (ABIN967652)

Bach, Szabo, Dighe, Ashkenazi, Aguet, Murphy, Schreiber: "Ligand-induced autoregulation of IFN-gamma receptor beta chain expression in T helper cell subsets." in: Science (New York, N.Y.), Vol. 270, Issue 5239, pp. 1215-8, (1996) (PubMed).

Target Details for IFNGR1

Target: IFNGR1 (Interferon gamma Receptor 1 (IFNGR1))

Alternative Name: CD119

Background: The 2E2 antibody recognizes the extracellular region of the 90 kDa alpha chain subunit of the mouse interferon-gamma receptor (IFN-gammaRalpha, aka, CD119). The functionally active-form of the mouse IFN-gamma receptor consists of two (or more) subunits, with IFN-gammaRalpha responsible for IFN-gamma binding and both the IFN-gammaRalpha and IFN-gammaRbeta chains required for the transduction of biologic responses. IFN-gammaRalpha is expressed by a variety of cell lines and normal mouse cells (except mature erythrocytes) including T cells, B cells, NK cells, monocytes, neutrophils, fibroblasts, epithelial and endothelial cells. The 2E2 antibody is a non-neutralizing antibody, it does not block the binding of IFN-gamma to its receptor. The immunogen used to generate this hybridoma was a purified preparation of soluble recombinant mouse IFN-gammaRalpha chain protein.
Synonyms: IFN-gamma Receptor alpha chain

Pathways: Interferon-gamma Pathway