ERK1 antibody (AA 325-345)

Catalog No. ABIN967701

Product Details anti-ERK1 Antibody

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Binding Specificity: AA 325-345

Reactivity: Human, Mouse, Rat, Chicken, Dog, Frog

Host: Mouse

Clonality: Monoclonal

Conjugate: This ERK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Cross-Reactivity: Mouse (Murine), Human, Chicken, Dog (Canine), Frog

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat ERK1 aa. 325-345

Clone: MK12

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-ERK1 antibody (ABIN967701)

Nowak: "Protein kinase C-alpha and ERK1/2 mediate mitochondrial dysfunction, decreases in active Na+ transport, and cisplatin-induced apoptosis in renal cells." in: The Journal of biological chemistry, Vol. 277, Issue 45, pp. 43377-88, (2002) (PubMed).

Aguirre-Ghiso, Liu, Mignatti, Kovalski, Ossowski: "Urokinase receptor and fibronectin regulate the ERK(MAPK) to p38(MAPK) activity ratios that determine carcinoma cell proliferation or dormancy in vivo." in: Molecular biology of the cell, Vol. 12, Issue 4, pp. 863-79, (2001) (PubMed).

Ackerley, Grierson, Brownlees, Thornhill, Anderton, Leigh, Shaw, Miller: "Glutamate slows axonal transport of neurofilaments in transfected neurons." in: The Journal of cell biology, Vol. 150, Issue 1, pp. 165-76, (2000) (PubMed).

Wan, Huang: "Analysis of the Gs/mitogen-activated protein kinase pathway in mutant S49 cells." in: The Journal of biological chemistry, Vol. 273, Issue 23, pp. 14533-7, (1998) (PubMed).

Reszka, Seger, Diltz, Krebs, Fischer: "Association of mitogen-activated protein kinase with the microtubule cytoskeleton." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 19, pp. 8881-5, (1995) (PubMed).

Target Details for ERK1

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Alternative Name: ERK1 (MAPK3 Products)

Background: The family of serine/threonine kinases known as ERKs (extracellular signal regulated kinases) or MAPKs (mitogen-activated protein kinases) is activated after cell stimulation by a variety of hormones and growth factors. Cell stimulation induces a signaling cascade that leads to phosphorylation of MEK (MAPK/ERK kinase) which, in turn, activates ERK via tyrosine and threonine phosphorylation. A myriad of proteins represent the downstream effectors for the active ERK and implicate it in the control of cell proliferation and differentiation, as well as regulation of the cytoskeleton. Activation of ERK is normally transient and cells possess dual specificity phosphatases that are responsible for its down-regulation. Furthermore, multiple studies have shown that elevated ERK activity is associated with some cancers. ERK1 is a 44 kDa member of the ERK family and shares 85% homology with ERK2 (42 kDa).

Molecular Weight: 44 kDa

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins