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ERK1 antibody

MAPK3 Reactivity: Rat WB, IHC, IF, FACS, ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN361833
  • Target See all ERK1 (MAPK3) Antibodies
    ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))
    Reactivity
    • 253
    • 175
    • 146
    • 45
    • 20
    • 19
    • 18
    • 12
    • 11
    • 11
    • 11
    • 7
    • 7
    • 6
    • 5
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    Rat
    Host
    • 262
    • 27
    • 4
    • 1
    • 1
    Rabbit
    Clonality
    • 238
    • 57
    Polyclonal
    Conjugate
    • 119
    • 25
    • 22
    • 20
    • 13
    • 11
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    This ERK1 antibody is un-conjugated
    Application
    • 247
    • 104
    • 85
    • 68
    • 65
    • 46
    • 41
    • 39
    • 34
    • 24
    • 18
    • 12
    • 12
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)
    Specificity
    Detects ~44kda (ERK1) and ~42 kDa (ERK2).
    Cross-Reactivity
    Chicken, Cow, Drosophila melanogaster, Human, Mouse, Rat, Sheep, Xenopus laevis
    Purification
    Peptide Affinity Purified
    Immunogen
    A 35 residue synthetic peptide, corresponding to Erk1 MAP kinase with the CGG spacer group added and the peptide coupled to KLH.
    Top Product
    Discover our top product MAPK3 Primary Antibody
  • Application Notes
    • WB (1:1000)
    • IHC (1:100)
    • ICC/IF (1:100)
    • FCM (1:100)
    • optimal dilutions for assays should be determined by the user.
    Comment

    A 1:1000 dilution of ABIN361832 was sufficient for detection of ERK1/2 in 20 μg of HeLa cell lysate by ECL immunoblot analysis.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    -20°C
  • Revuelta-López, Cal, Herraiz-Martínez, de Gonzalo-Calvo, Nasarre, Roura, Gálvez-Montón, Bayes-Genis, Badimon, Hove-Madsen, Llorente-Cortés: "Hypoxia-driven sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (SERCA2) downregulation depends on low-density lipoprotein receptor-related protein 1 (LRP1)-signalling in cardiomyocytes." in: Journal of molecular and cellular cardiology, Vol. 85, pp. 25-36, (2015) (PubMed).

    Thon, Hosoi, Yoshii, Ozawa: "Leptin induced GRP78 expression through the PI3K-mTOR pathway in neuronal cells." in: Scientific reports, Vol. 4, pp. 7096, (2014) (PubMed).

    Bhat, Gomis, Potter, Tikoo: "Role of Hsp90 in CpG ODN mediated immunostimulation in avian macrophages." in: Molecular immunology, Vol. 47, Issue 6, pp. 1337-46, (2010) (PubMed).

    Enikanolaiye, Larivière, Troy, Arabzadeh, Atasoy, Turksen: "Involucrin-claudin-6 tail deletion mutant (CDelta206) transgenic mice: a model of delayed epidermal permeability barrier formation and repair." in: Disease models & mechanisms, Vol. 3, Issue 3-4, pp. 167-80, (2010) (PubMed).

  • Target
    ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))
    Alternative Name
    ERK1 (MAPK3 Products)
    Synonyms
    ERK-1 antibody, ERK1 antibody, ERT2 antibody, HS44KDAP antibody, HUMKER1A antibody, P44ERK1 antibody, P44MAPK antibody, PRKM3 antibody, p44-ERK1 antibody, p44-MAPK antibody, Erk-1 antibody, Erk1 antibody, Ert2 antibody, Esrk1 antibody, Mnk1 antibody, Mtap2k antibody, Prkm3 antibody, p44 antibody, p44erk1 antibody, p44mapk antibody, ERK3 antibody, ERK6 antibody, P38GAMMA antibody, PRKM12 antibody, SAPK-3 antibody, SAPK3 antibody, fi06b09 antibody, wu:fi06b09 antibody, zERK1 antibody, Tb08.10J17.940 antibody, MAPK1 antibody, MNK1 antibody, AW123708 antibody, Erk6 antibody, P38gamma antibody, Prkm12 antibody, Sapk3 antibody, ATMAPK3 antibody, ATMPK3 antibody, T6D9.4 antibody, mitogen-activated protein kinase 3 antibody, mitogen-activated protein kinase 3 antibody, mitogen-activated protein kinase 12 antibody, mitogen activated protein kinase 3 antibody, mitogen-activated serine/threonine-protein kinase antibody, MAPK3 antibody, Mapk3 antibody, MAPK12 antibody, mapk3 antibody, Tc00.1047053509475.10 antibody, Tb927.8.3550 antibody, Mapk12 antibody, CEK1 antibody, MPK3 antibody
    Background
    The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase (1). They are both located in the cytosol and mitochondria (2). While the role of cytosol ERK1/2 is well studied and involved in multiple cellular functions (2), the role of mitochondrial ERK1/2 remains poorly understood. Both ERK 1 and 2 are activated by MEK1 or MEK2, by dual phosphorylation of a threonine and tyrosine residue in the activation loop (TEY motif) (1, 3). Either phosphorylation alone can induce an electrophoretic mobility shift, but both are required for activation of the kinase. This dual phosphorylation is efficiently detected by phosphorylation state-specific antibody directed to the pTEpY motif. Once activated, MAP kinases phosphorylate a broad spectrum of substrates, including cytoskeletal proteins, translation regulators, transcription factors, and the Rsk family of protein kinases (4). ERK1/2 activation is generally thought to confer a survival advantage to cells (5), however there is increasing evidence that suggests that the activation of ERK1/2 also contributes to cell death under certain conditions (5). ERK1/2 also is activated in neuronal and renal epithelial cells upon exposure to oxidative stress and toxicants or deprivation of growth factors, and inhibition of the ERK pathway blocks apoptosis (5).
    Gene ID
    50689
    NCBI Accession
    NP_059043
    UniProt
    P21708
    Pathways
    MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins
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