CAMK4 antibody (AA 1-241)

Catalog No. ABIN967876

This Mouse Monoclonal antibody specifically detects CAMK4 in WB, IHC, IF and IP. It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 5+ publications.

Quick Overview for CAMK4 antibody (AA 1-241) (ABIN967876)

Target: CAMK4 (Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CAMK4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 26-CaM Kinase IV

Product Details anti-CAMK4 Antibody

Binding Specificity: AA 1-241

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human CaM Kinase IV aa. 1-241

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-CAMK4 antibody (ABIN967876)

Wei, Qiu, Liauw, Robinson, Ho, Chatila, Zhuo: "Calcium calmodulin-dependent protein kinase IV is required for fear memory." in: Nature neuroscience, Vol. 5, Issue 6, pp. 573-9, (2002) (PubMed).

Slee, Adrain, Martin: "Executioner caspase-3, -6, and -7 perform distinct, non-redundant roles during the demolition phase of apoptosis." in: The Journal of biological chemistry, Vol. 276, Issue 10, pp. 7320-6, (2001) (PubMed).

Passier, Zeng, Frey, Naya, Nicol, McKinsey, Overbeek, Richardson, Grant, Olson: "CaM kinase signaling induces cardiac hypertrophy and activates the MEF2 transcription factor in vivo." in: The Journal of clinical investigation, Vol. 105, Issue 10, pp. 1395-406, (2000) (PubMed).

Wayman, Walters, Kolibaba, Soderling, Christian: "CaM kinase IV regulates lineage commitment and survival of erythroid progenitors in a non-cell-autonomous manner." in: The Journal of cell biology, Vol. 151, Issue 4, pp. 811-24, (2000) (PubMed).

Ohmstede, Bland, Merrill, Sahyoun: "Relationship of genes encoding Ca2+/calmodulin-dependent protein kinase Gr and calspermin: a gene within a gene." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 13, pp. 5784-8, (1991) (PubMed).

Target Details for CAMK4

Target: CAMK4 (Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4))

Alternative Name: CaM Kinase IV

Background: CaM-kinase IV (Ca2+/calmodulin-dependent protein kinase IV, also described as CaM-kinase Gr) is activated through the binding of Ca2+/CaM and by phosphorylation. This kinase has high sequence homologies with the catalytic and regulatory domains of CaM-kinase II. CaM-kinase IV is a monomer which is highly expressed in cerebellum, thymus, and testis. Its subcellular distrubution includes localization in both the synaptic regions of the molecular layer of the cerebellum, and the nuclei of cerebellar granule cells. CaM-kinase IV has an autoinhibitory domain within residues 305-321 that can suppress kinase activity in the absence of Ca2+/CaM. This type of domain is common in many other CaM-dependent kinases and phosphatases. CaM-kinase IV does not appear to be significantly activated by autophosphorylation, but it can be activated approximately 5-10-fold when phosphorylated by CaM-kinase IV kinase.

Molecular Weight: 60 kDa

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Production of Molecular Mediator of Immune Response, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma