RAB5 antibody (AA 1-215)

Catalog No. ABIN967882

This Mouse Monoclonal antibody specifically detects RAB5 in WB, IHC, IF and IP. It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 5+ publications.

Quick Overview for RAB5 antibody (AA 1-215) (ABIN967882)

Target: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This RAB5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 15-Rab5

Product Details anti-RAB5 Antibody

Binding Specificity: AA 1-215

Cross-Reactivity: Rat (Rattus), Mouse (Murine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Rab5 aa. 1-215

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968536, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-RAB5 antibody (ABIN967882)

Huang, Imamura, Olefsky: "Insulin can regulate GLUT4 internalization by signaling to Rab5 and the motor protein dynein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 23, pp. 13084-9, (2001) (PubMed).

Wang, Hu, Zimmermann, Kilimann: "Rim1 and rabphilin-3 bind Rab3-GTP by composite determinants partially related through N-terminal alpha -helix motifs." in: The Journal of biological chemistry, Vol. 276, Issue 35, pp. 32480-8, (2001) (PubMed).

Pinkoski, Hobman, Heibein, Tomaselli, Li, Seth, Froelich, Bleackley: "Entry and trafficking of granzyme B in target cells during granzyme B-perforin-mediated apoptosis." in: Blood, Vol. 92, Issue 3, pp. 1044-54, (1998) (PubMed).

Li, Stahl: "Structure-function relationship of the small GTPase rab5." in: The Journal of biological chemistry, Vol. 268, Issue 32, pp. 24475-80, (1993) (PubMed).

Sanford, Pan, Wessling-Resnick: "Prenylation of Rab5 is dependent on guanine nucleotide binding." in: The Journal of biological chemistry, Vol. 268, Issue 32, pp. 23773-6, (1993) (PubMed).

Target Details for RAB5

Target: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Alternative Name: Rab5

Background: Rab5 is a low molecular weight GTP-binding protein that plays a role in endocytic vesicle traffic. Like other Rab proteins, Rab5 has C-terminal cysteine residues that are post-translationally modified by geranylgeranylation, which is critical for its membrane targeting. Rab5 is associated with early endosome and plasma membranes and evidence suggests that Rab5 is involved in regulation of early endosome fusion. The GTP/GDP cycle controls shuttling of Rab proteins between the cytosol and organelle membranes. In vitro, Rab5 proteins are removed from membranes by a GDP dissociation inhibitor protein (rabGDI) which leads to the formation of a cytosolic Rab5-rabGDI complex. Rab5 insertion into membranes is a multistep process in which a transient GDP-Rab5 intermediate is formed and converted into GTP-Rab5 that subsequently enters the acceptor membrane and releases rabGDI into the cytosol.

Molecular Weight: 25 kDA

Pathways: Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity