RAN antibody (AA 7-171)

Catalog No. ABIN967913

The Mouse Monoclonal anti-RAN antibody has been validated for WB, IHC, IF and IP. It is suitable to detect RAN in samples from Human, Mouse, Rat, Dog and Chicken. There are 5+ publications available.

Quick Overview for RAN antibody (AA 7-171) (ABIN967913)

Target: RAN (RAN, Member RAS Oncogene Family (RAN))

Reactivity: Human, Mouse, Rat, Dog, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This RAN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 20-Ran

Product Details anti-RAN Antibody

Binding Specificity: AA 7-171

Cross-Reactivity: Chicken, Dog (Canine), Mouse (Murine), Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Ran aa. 7-171

Isotype: IgG2a

Application Details

Comment:

Related Products: ABIN968533, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-RAN antibody (ABIN967913)

Nemergut, Lindsay, Brownawell, Macara: "Ran-binding protein 3 links Crm1 to the Ran guanine nucleotide exchange factor." in: The Journal of biological chemistry, Vol. 277, Issue 20, pp. 17385-8, (2002) (PubMed).

Schwoebel, Ho, Moore: "The mechanism of inhibition of Ran-dependent nuclear transport by cellular ATP depletion." in: The Journal of cell biology, Vol. 157, Issue 6, pp. 963-74, (2002) (PubMed).

Faleiro, Lazebnik: "Caspases disrupt the nuclear-cytoplasmic barrier." in: The Journal of cell biology, Vol. 151, Issue 5, pp. 951-9, (2000) (PubMed).

Iborra, Jackson, Cook: "The path of RNA through nuclear pores: apparent entry from the sides into specialized pores." in: Journal of cell science, Vol. 113 Pt 2, pp. 291-302, (2000) (PubMed).

Drivas, Shih, Coutavas, Rush, DEustachio: "Characterization of four novel ras-like genes expressed in a human teratocarcinoma cell line." in: Molecular and cellular biology, Vol. 10, Issue 4, pp. 1793-8, (1990) (PubMed).

Target Details for RAN

Target: RAN (RAN, Member RAS Oncogene Family (RAN))

Alternative Name: Ran

Background: Ran is a highly conserved GTPase that is ubiquitously expressed. At steady-state, 80-90% of cellular Ran is located in the nucleus, with the remainder in the cytoplasm. The human TC4/Ran cDNA was originally isolated based on the predicted homology of its encoded protein product to Ras. Unlike other members of the Ras superfamily, Ran does not appear to contain signals for lipid modification, nor does it appear to show membrane localization. Ran has been implicated in a number of cellular processes such as the initiation of DNA replication, entry into and exit from mitosis and in nuclear RNA and protein transport through the nuclear pore complex. A number of Ran binding proteins have been identified. One of the smaller proteins identified is a 28kDa cytosolic molecule known as RanBP1. The GTP-bound state of Ran is stabilized through its binding to RanBP1.

Molecular Weight: 25 kDa

Pathways: Regulatory RNA Pathways, Intracellular Steroid Hormone Receptor Signaling Pathway, Protein targeting to Nucleus