HSP90 antibody (AA 586-732)

Catalog No. ABIN967958

The Mouse Monoclonal anti-HSP90 antibody has been validated for WB, IP, IHC and IF. It is suitable to detect HSP90 in samples from Human, Rat, Mouse, Chicken and Dog. There are 6+ publications available.

Quick Overview for HSP90 antibody (AA 586-732) (ABIN967958)

Target: HSP90 (Heat Shock Protein 90 (HSP90))

Reactivity: Human, Rat, Mouse, Chicken, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSP90 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: 68-Hsp90

Product Details anti-HSP90 Antibody

Binding Specificity: AA 586-732

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Dog (Canine), Chicken

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Hsp90 aa. 586-732

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-HSP90 antibody (ABIN967958)

Murata, Suzuki, Yamawaki, Sato, Hori, Karaki, Ozaki: "Dexamethasone prevents impairment of endothelium-dependent relaxation in arteries cultured with fetal bovine serum." in: European journal of pharmacology, Vol. 515, Issue 1-3, pp. 134-41, (2005) (PubMed).

Miyamoto, Nakayama, Imaki, Hirose, Jiang, Abe, Tsukiyama, Nagahama, Ohno, Hatakeyama, Nakayama: "Increased proliferation of B cells and auto-immunity in mice lacking protein kinase Cdelta." in: Nature, Vol. 416, Issue 6883, pp. 865-9, (2002) (PubMed).

Murata, Sato, Hori, Ozaki, Karaki: "Decreased endothelial nitric-oxide synthase (eNOS) activity resulting from abnormal interaction between eNOS and its regulatory proteins in hypoxia-induced pulmonary hypertension." in: The Journal of biological chemistry, Vol. 277, Issue 46, pp. 44085-92, (2002) (PubMed).

Brouet, Sonveaux, Dessy, Balligand, Feron: "Hsp90 ensures the transition from the early Ca2+-dependent to the late phosphorylation-dependent activation of the endothelial nitric-oxide synthase in vascular endothelial growth factor-exposed endothelial cells." in: The Journal of biological chemistry, Vol. 276, Issue 35, pp. 32663-9, (2001) (PubMed).

Pritchard, Ackerman, Gross, Stepp, Shi, Fontana, Baker, Sessa: "Heat shock protein 90 mediates the balance of nitric oxide and superoxide anion from endothelial nitric-oxide synthase." in: The Journal of biological chemistry, Vol. 276, Issue 21, pp. 17621-4, (2001) (PubMed).

Rebbe, Ware, Bertina, Modrich, Stafford: "Nucleotide sequence of a cDNA for a member of the human 90-kDa heat-shock protein family." in: Gene, Vol. 53, Issue 2-3, pp. 235-45, (1987) (PubMed).

Target Details for HSP90

Target: HSP90 (Heat Shock Protein 90 (HSP90))

Alternative Name: Hsp90

Background: The 90 kDa heat shock protein (Hsp90), a highly conserved stress-induced protein, is abundantly expressed in most tissues under nonstress conditions and is required for eukaryotic cell viability. Hsp90 interacts with a number of signaling molecules, other heat shock proteins, and cytoskeletal proteins. However, the function of Hsp90 in these complexes remains unclear. It is thought that Hsp90 plays a common role in a number of diverse signaling systems due to its physical association with v-Src family tyrosine kinases, steroid hormone receptors, the Raf serine/threonine kinase, the basic-helix-loop-helix dioxin receptor, and the betagamma subunits of G proteins.
Synonyms: Heat Shock Protein-90

Molecular Weight: 90 kDa

Pathways: M Phase, Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals