Dynactin 1 antibody (AA 3-202)

Catalog No. ABIN967996

This Mouse Monoclonal antibody specifically detects Dynactin 1 in WB, IF, IP and IHC (zinc). It exhibits reactivity toward Human, Mouse, Rat, Dog and Chicken and has been mentioned in 5+ publications.

Quick Overview for Dynactin 1 antibody (AA 3-202) (ABIN967996)

Target: Dynactin 1 (DCTN1)

Reactivity: Human, Mouse, Rat, Dog, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Dynactin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))

Clone: 1-p150Glued

Product Details anti-Dynactin 1 Antibody

Binding Specificity: AA 3-202

Cross-Reactivity: Human, Chicken, Dog (Canine), Mouse (Murine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat p150 [Glued] aa. 3-202

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968536, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-Dynactin 1 antibody (ABIN967996)

Kurzchalia: "Anthrax toxin rafts into cells." in: The Journal of cell biology, Vol. 160, Issue 3, pp. 295-6, (2003) (PubMed).

Askham, Vaughan, Goodson, Morrison: "Evidence that an interaction between EB1 and p150(Glued) is required for the formation and maintenance of a radial microtubule array anchored at the centrosome." in: Molecular biology of the cell, Vol. 13, Issue 10, pp. 3627-45, (2002) (PubMed).

Aumais, Tunstead, McNeil, Schaar, McConnell, Lin, Clark, Yu-Lee: "NudC associates with Lis1 and the dynein motor at the leading pole of neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 21, Issue 24, pp. RC187, (2001) (PubMed).

Tai, Chuang, Sung: "Cytoplasmic dynein regulation by subunit heterogeneity and its role in apical transport." in: The Journal of cell biology, Vol. 153, Issue 7, pp. 1499-509, (2001) (PubMed).

King, Schroer: "Dynactin increases the processivity of the cytoplasmic dynein motor." in: Nature cell biology, Vol. 2, Issue 1, pp. 20-4, (2000) (PubMed).

Target Details for Dynactin 1

Target: Dynactin 1 (DCTN1)

Alternative Name: p150 Glued

Background: P150 [Glued] was identified as a polypeptide associated with cytoplasmic dynein, the minus-end-directed microtubule-based motor protein. p150 [Glued] is also a member of the oligomeric dynactin complex. Dynactin mediates dynein-driven vesicle motility, as well as lower eukaryote nuclear transport. p150 [Glued] bears significant homology to the product of the Glued gene in Drosophila. It has been shown in vitro to be a required activator of dynein-mediated transport along microtubules. The p150 [Glued] component of the dynactin complex binds to microtubules and the actin-like protein Centractin (Arp-1), another member of the dynactin complex. In the developing rat, p150 [Glued] is expressed at high levels in neural tissue. Microtubule bindings assays with selected constructs of p150 [Glued] indicate that amino acids 39-150 are required for microtubule association. This antibody is routinely tested by western blot analysis.

Molecular Weight: 150 kDa

Pathways: M Phase, ER-Nucleus Signaling