TRIM25 antibody (AA 32-210)

Catalog No. ABIN968044

This Mouse Monoclonal antibody specifically detects TRIM25 in WB, IHC, IF and IP. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for TRIM25 antibody (AA 32-210) (ABIN968044)

Target: TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TRIM25 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 2-EFP

Product Details anti-TRIM25 Antibody

Binding Specificity: AA 32-210

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human EFP aa. 32-210

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968536, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-TRIM25 antibody (ABIN968044)

Inoue, Orimo, Hosoi, Kondo, Toyoshima, Kondo, Ikegami, Ouchi, Orimo, Muramatsu: "Genomic binding-site cloning reveals an estrogen-responsive gene that encodes a RING finger protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 23, pp. 11117-21, (1994) (PubMed).

Target Details for TRIM25

Target: TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Alternative Name: EFP

Background: Steriod hormones, unlike polypeptide hormones, diffuse inside the cell, bind cytoplasmic or nuclear receptors, and activate specific genes. Complete identification of such receptors would greatly increase the understanding of the celluar processes activated by steriods. EFP (Estrogen receptor Finger Protein) was identified as a result of its specific interaction with the estrogen receptor. EFP contains a zinc-finger domain, indicating that it is a DNA-binding protein. EFP transcription is regulated by estrogens and, in combination with the estrogen receptor, it may modulate the expression of estrogen-responsive genes. This antibody is routinely tested by western blot analysis.
Synonyms: Estrogen receptor Finger Protein

Molecular Weight: 71 kDa