HSP70 antibody (AA 429-640)

Catalog No. ABIN968060

The Mouse Monoclonal anti-HSP70 antibody has been validated for WB, IHC, IF and IP. It is suitable to detect HSP70 in samples from Human, Mouse, Rat, Chicken and Dog. There are 2+ publications available.

Quick Overview for HSP70 antibody (AA 429-640) (ABIN968060)

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Reactivity: Human, Mouse, Rat, Chicken, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSP70 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 7-Hsp70

Product Details anti-HSP70 Antibody

Binding Specificity: AA 429-640

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Chicken, Dog (Canine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Hsp70 aa. 429-640

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-HSP70 antibody (ABIN968060)

Suhr, Senut, Whitelegge, Faull, Cuizon, Gage: "Identities of sequestered proteins in aggregates from cells with induced polyglutamine expression." in: The Journal of cell biology, Vol. 153, Issue 2, pp. 283-94, (2001) (PubMed).

Hunt, Morimoto: "Conserved features of eukaryotic hsp70 genes revealed by comparison with the nucleotide sequence of human hsp70." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, Issue 19, pp. 6455-9, (1985) (PubMed).

Target Details for HSP70

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Alternative Name: Hsp70

Background: Hsp70 is a member of the heat shock or stress-induced genes whose expression is regulated at both transcriptional and translational levels. Elevated temperatures induce a transient resistance to subsequent heat exposure in various types of cells. Although Hsp70 protein is constitutively expressed at low levels, its expression abundantly increases in response to heat stress. During such stress, Hsp70 leaves the cytoplasm and nucleoplasm and rapidly accumulates in the nucleolus. Removal of the stress results in the redistribution of Hsp70 to its original locations. This antibody is routinely tested by western blot analysis.

Molecular Weight: 70 kDa