ITGA5 antibody (AA 853-1002)

Catalog No. ABIN968077

This Mouse Monoclonal antibody specifically detects ITGA5 in WB, IHC, IP and IF. It exhibits reactivity toward Human and has been mentioned in 5+ publications.

Quick Overview for ITGA5 antibody (AA 853-1002) (ABIN968077)

Target: ITGA5 (Integrin, alpha 5 (ITGA5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGA5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: 1-CD49e

Product Details anti-ITGA5 Antibody

Binding Specificity: AA 853-1002

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Integrin alpha5 aa. 853-1002

Isotype: IgG2a

Application Details

Comment:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-ITGA5 antibody (ABIN968077)

Sottile, Hocking: "Fibronectin polymerization regulates the composition and stability of extracellular matrix fibrils and cell-matrix adhesions." in: Molecular biology of the cell, Vol. 13, Issue 10, pp. 3546-59, (2002) (PubMed).

Alahari, Lee, Juliano: "Nischarin, a novel protein that interacts with the integrin alpha5 subunit and inhibits cell migration." in: The Journal of cell biology, Vol. 151, Issue 6, pp. 1141-54, (2001) (PubMed).

Aplin, Short, Juliano: "Anchorage-dependent regulation of the mitogen-activated protein kinase cascade by growth factors is supported by a variety of integrin alpha chains." in: The Journal of biological chemistry, Vol. 274, Issue 44, pp. 31223-8, (1999) (PubMed).

Zheng, Fang, Hakomori et al.: "Functional role of N-glycosylation in alpha 5 beta 1 integrin receptor. De-N-glycosylation induces dissociation or altered association of alpha 5 and beta 1 subunits and concomitant loss of ..." in: The Journal of biological chemistry, Vol. 269, Issue 16, pp. 12325-31, (1994) (PubMed).

Bauer, Varner, Schreiner, Kornberg, Nicholas, Juliano: "Functional role of the cytoplasmic domain of the integrin alpha 5 subunit." in: The Journal of cell biology, Vol. 122, Issue 1, pp. 209-21, (1993) (PubMed).

Target Details for ITGA5

Target: ITGA5 (Integrin, alpha 5 (ITGA5))

Alternative Name: CD49e

Background: Integrins are membrane receptors that mediate cell-cell or cell-matrix adhesion. They are transmembrane heterodimers composed of alpha and beta subunits and are connected to the cytoskeleton. At least 20 integrins, formed from combinations of 12 alpha and 9 beta subunits, have been reported. Many of these have been implicated as transducers of molecular signals. The integrin alpha5 subunit is 1049 amino acids with a molecular weight of 150-165 kDa. alpha5 and beta1 form a functional fibronectin receptor which is important for vascular development in mice. Both subunits of the fibronectin receptor are heavily glycosylated in their extracellular domains. This modification is essential for proper cell attachment to basal membranes. Integrin alpha5 has been reported to suppress apoptosis by a Bcl-2 pathway and the C-terminal region is critical for cell motility and cytoskeletal rearrangements. This antibody is routinely tested by western blot analysis.
Synonyms: Integrin alpha5

Molecular Weight: 150 kDa

Pathways: Integrin Complex