NINJ1 antibody (AA 1-152)

Catalog No. ABIN968172

This anti-NINJ1 antibody is a Mouse Monoclonal antibody detecting NINJ1 in WB, IHC, IF and IP. Suitable for Human, Mouse, Rat and Dog. This Primary Antibody has been cited in 3+ publications.

Quick Overview for NINJ1 antibody (AA 1-152) (ABIN968172)

Target: NINJ1 (Ninjurin 1 (NINJ1))

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This NINJ1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 50-Ninjurin

Product Details anti-NINJ1 Antibody

Binding Specificity: AA 1-152

Cross-Reactivity: Dog (Canine), Rat (Rattus), Mouse (Murine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Ninjurin aa. 1-152

Isotype: IgG2a

Application Details

Comment:

Related Products: ABIN968587, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-NINJ1 antibody (ABIN968172)

Chen, Coustan-Smith, Suzuki, Neale, Mihara, Pui, Campana: "Identification of novel markers for monitoring minimal residual disease in acute lymphoblastic leukemia." in: Blood, Vol. 97, Issue 7, pp. 2115-20, (2001) (PubMed).

Araki, Zimonjic, Popescu, Milbrandt: "Mechanism of homophilic binding mediated by ninjurin, a novel widely expressed adhesion molecule." in: The Journal of biological chemistry, Vol. 272, Issue 34, pp. 21373-80, (1997) (PubMed).

Araki, Milbrandt: "Ninjurin, a novel adhesion molecule, is induced by nerve injury and promotes axonal growth." in: Neuron, Vol. 17, Issue 2, pp. 353-61, (1996) (PubMed).

Target Details for NINJ1

Target: NINJ1 (Ninjurin 1 (NINJ1))

Alternative Name: Ninjurin

Background: Ninjurin is a protein whose expression is dramatically increased after sciatic nerve transection and crush injuries. The Ninjurin gene encodes for a protein of 152 amino acids with two putative transmembrane domains. Ninjurin was iodinated in vivo and promoted the aggregation of Jurkat cells expressing Ninjurin, indicating a portion of Ninjurin is exposed to the cell surface. The adhesive properties of Ninjurin were energy and temperature dependent, required Ca2+ and Mg2+, and the integrity of the cytoskeleton. Also, the adhesion domain of Ninjurin is located at the extracellular NH2-terminal domain. Although its predicted mass is 16 kDa, Ninjurin migrates as a 18-22 kDa protein in SDS-PAGE, depending on the cell line or tissue. mRNA analysis revealed that Ninjurin is widely expressed with the highest levels in liver, thymus, heart, and the lowest level in brain.

Molecular Weight: 18-22 kDa