SSB antibody (AA 179-289)

Catalog No. ABIN968242

This Mouse Monoclonal antibody specifically detects SSB in WB and IF. It exhibits reactivity toward Human, Rat, Dog and Rabbit and has been mentioned in 3+ publications.

Quick Overview for SSB antibody (AA 179-289) (ABIN968242)

Target: SSB (Sjogren Syndrome Antigen B (SSB))

Reactivity: Human, Rat, Dog, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This SSB antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 44-La Protein

Product Details anti-SSB Antibody

Binding Specificity: AA 179-289

Cross-Reactivity: Human, Dog (Canine), Rabbit, Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human La Protein aa. 179-289

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968551, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-SSB antibody (ABIN968242)

Goodier, Fan, Maraia: "A carboxy-terminal basic region controls RNA polymerase III transcription factor activity of human La protein." in: Molecular and cellular biology, Vol. 17, Issue 10, pp. 5823-32, (1997) (PubMed).

McLaren, Caruccio, Ross: "Human La protein: a stabilizer of histone mRNA." in: Molecular and cellular biology, Vol. 17, Issue 6, pp. 3028-36, (1997) (PubMed).

Chambers, Kenan, Martin, Keene: "Genomic structure and amino acid sequence domains of the human La autoantigen." in: The Journal of biological chemistry, Vol. 263, Issue 34, pp. 18043-51, (1989) (PubMed).

Target Details for SSB

Target: SSB (Sjogren Syndrome Antigen B (SSB))

Alternative Name: La Protein

Background: La was first found to bind autoantibodies from individuals with Sjogren's syndrome and systemic lupus erythematosus (SLE). La is a transcription factor for RNA polymerase III (Pol III). It participates in the termination of Pol III-mediated transcription and the recycling of the transcriptional machinery. La associates with the 3' oligo U portion of transcripts generated by Pol III. In some cases, this association protects the message from 3' processing. The association of La and mRNA is mediated by the presence of an N-terminal ribonucleoprotein (RNP) consensus sequence in La. The La protein also binds several small RNAs, such as 7S RNA, 5S RNA, and tRNA, and is thought to be involved in their biogenesis. It is known that histone mRNA is stabilized throughout S Phase and is degraded only at the end of S phase. La protein participates in the stabilization of histone mRNA which results in increased production of histone protein. These data suggest that a major function of La is to protect vital nascent transcripts from premature degradation.

Molecular Weight: 47 kDa