CG7626 Gene Product From Transcript CG7626-RA (SPT5) (AA 866-985) antibody

Catalog No. ABIN968370

Cited in 1 publication. The Mouse Monoclonal anti-CG7626 Gene Product From Transcript CG7626-RA antibody (Clone 17-DSIF) (ABIN968370) specifically detects CG7626 Gene Product From Transcript CG7626-RA in IF and WB. The antibody is reactive with Dog, Human, Mouse and Rat samples.

Quick Overview for CG7626 Gene Product From Transcript CG7626-RA (SPT5) (AA 866-985) antibody (ABIN968370)

Target: CG7626 Gene Product From Transcript CG7626-RA (SPT5)

Reactivity: Dog, Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Application: Immunofluorescence (IF), Western Blotting (WB)

Clone: 17-DSIF

Product Details

Binding Specificity: AA 866-985

Cross-Reactivity: Dog (Canine), Mouse (Murine), Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human DSIF aa. 866-985

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References

Kim, Bourgeois, Isel, Churcher, Karn: "Phosphorylation of the RNA polymerase II carboxyl-terminal domain by CDK9 is directly responsible for human immunodeficiency virus type 1 Tat-activated transcriptional elongation." in: Molecular and cellular biology, Vol. 22, Issue 13, pp. 4622-37, (2002) (PubMed).

Target Details

Target: CG7626 Gene Product From Transcript CG7626-RA (SPT5)

Alternative Name: DSIF

Background: In living systems, the relative amounts of any protein are controlled at many levels. For example, amounts are affected by protein degradation, regulation of the translational rates of polypeptide synthesis (translational regulation), and control of the rates of mRNA synthesis (transcriptional regulation). Transcriptional regulation involves modulation of the rate-limiting enzyme RNA polymerase. DSIF (DRB sensitivity-inducing factor) is a heterodimeric transcription elongation protein. It is composed of a large subunit of 160 kDa and a small subunit of 14 kDa. These large and the small subunits are homologs of the yeast gene products Stp5 and Stp4, respectively. Spt4 and 5 are transcription factors which are critically important for the activity of RNA polymerase. In conjunction with DRB, DSIF attenuates RNA polymerase II elongation steps. However, in limiting amounts of ribonucleotides, DSIF, by itself, stimulates the elongation rate of RNA polymerase II. Thus, the identification of a human regulator for transcriptional elongation will greatly enhance our understanding of this critical step in mammalian gene expression. This antibody is routinely tested by western blot analysis.

Molecular Weight: 160 kDa