HDAC3 antibody (AA 309-425)

Catalog No. ABIN968378

The Mouse Monoclonal anti-HDAC3 antibody has been validated for WB and IF. It is suitable to detect HDAC3 in samples from Human, Mouse, Rat and Dog.

Quick Overview for HDAC3 antibody (AA 309-425) (ABIN968378)

Target: HDAC3 (Histone Deacetylase 3 (HDAC3))

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This HDAC3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 40-HDAC3

Product Details anti-HDAC3 Antibody

Binding Specificity: AA 309-425

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Dog (Canine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human HDAC3 aa. 309-425

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968536, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

Target Details for HDAC3

Target: HDAC3 (Histone Deacetylase 3 (HDAC3))

Alternative Name: HDAC3

Background: Regulation of gene expression occurs at multiple levels and is mediated by numerous factors. DNA-binding proteins such as histones function to spatially organize the DNA into chromatin. The spatial organization of chromatin is also important for gene expression, and modifications to DNA-binding proteins have profound effects on gene expression. Human Histone Deacetylase 3 (HDAC3) is a protein of approximately 50 kDa that removes acetyl groups from the epsilon-amino groups of lysines in histones, thus in competition with the acetylases. HDAC3 is the third member of this family of deacetylases, related to the yeast RPD3 gene, which affect transcription of certain human genes. HDAC3 importance is reflected in its distribution in virtually every cell type examined. In in vitro assays, HDAC3 exhibited activity towards histones H3 and H4 acetylated in vivo. Furthermore, inhibitors of deacetylation, such as sodium butyrate, inhibited HDAC3 activity. Further studies will determine the specific role of HDAC3 in regulating gene expression.
Synonyms: Histone Deacetylase-3

Molecular Weight: 50 kDa

Pathways: Neurotrophin Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development