Ataxin 2 antibody (AA 713-904)

Catalog No. ABIN968504

The Mouse Monoclonal anti-Ataxin 2 antibody has been validated for WB and IF. It is suitable to detect Ataxin 2 in samples from Human and Rat. There are 3+ publications available.

Quick Overview for Ataxin 2 antibody (AA 713-904) (ABIN968504)

Target: Ataxin 2 (ATXN2)

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ataxin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 22-Ataxin

Product Details anti-Ataxin 2 Antibody

Binding Specificity: AA 713-904

Cross-Reactivity: Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Ataxin-2 aa. 713-904

Isotype: IgG1

Application Details

Application Notes: Methanol Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 90% methanol. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Wash three times with PBS. Flick out PBS and add second step reagent. Incubate for 1 hour at RT. Wash three times with PBS.
Triton-X 100 Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 0.1% Triton-X 100. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Flick out and wash three times with PBS. Flick out and add second step reagent. Incubate for 1 hour at RT. Flick out and wash three times with PBS.

Comment:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-Ataxin 2 antibody (ABIN968504)

Huynh, Del Bigio, Ho, Pulst: "Expression of ataxin-2 in brains from normal individuals and patients with Alzheimer's disease and spinocerebellar ataxia 2." in: Annals of neurology, Vol. 45, Issue 2, pp. 232-41, (1999) (PubMed).

Nechiporuk, Huynh, Figueroa, Sahba, Nechiporuk, Pulst: "The mouse SCA2 gene: cDNA sequence, alternative splicing and protein expression." in: Human molecular genetics, Vol. 7, Issue 8, pp. 1301-9, (1998) (PubMed).

Pulst, Nechiporuk, Nechiporuk, Gispert, Chen, Lopes-Cendes, Pearlman, Starkman, Orozco-Diaz, Lunkes, DeJong, Rouleau, Auburger, Korenberg, Figueroa, Sahba: "Moderate expansion of a normally biallelic trinucleotide repeat in spinocerebellar ataxia type 2." in: Nature genetics, Vol. 14, Issue 3, pp. 269-76, (1996) (PubMed).

Target Details for Ataxin 2

Target: Ataxin 2 (ATXN2)

Alternative Name: Ataxin-2

Background: The hereditary ataxias are neurodegenerative disorders characterized by abnormalities of balance due to dysfunction of the cerebellum and cerebellar pathways. Spinocerebellar ataxias (SCAs) represent a heterogeneous group of disorders with a prevalence of about 1 in 10^5. Moderate expansion (36 or 37 units) of CAG repeats on the SCA2 gene leads to a gene product, ataxin-2, that has a long polyglutamine tract. Ataxin-2 is a basic protein with two domains (Sm1 and Sm2) that have been implicated in RNA splicing and protein interaction. Human ataxin-2 has significant homology with mouse ataxin-2 and ataxin-2 related protein. Ataxin-2 is expressed as early as day 8 of mouse embryogenesis and is detected in brain, heart, placenta, liver, skeletal muscle, and pancreas, but not in lung or kidney. Expression increases with age and the protein is localized to the cytoplasm in purkinje cells and other neuronal cell types. Thus, ataxin-2 is thought to be important for RNA splicing or protein complex formation in many normal tissues, while polyglutamine containing ataxin-2 leads to neuropathology.

Molecular Weight: 150 kDa

Pathways: Ribonucleoprotein Complex Subunit Organization