GOLGA5 antibody (AA 510-713)

Catalog No. ABIN968506

This Mouse Monoclonal antibody specifically detects GOLGA5 in WB and IF. It exhibits reactivity toward Human and Dogand has been mentioned in 1 publication.

Quick Overview for GOLGA5 antibody (AA 510-713) (ABIN968506)

Target: GOLGA5 (Golgin A5 (GOLGA5))

Reactivity: Human, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This GOLGA5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 26-Golgin

Product Details anti-GOLGA5 Antibody

Binding Specificity: AA 510-713

Cross-Reactivity: Dog (Canine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Golgin-84 aa. 510-713

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968552, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-GOLGA5 antibody (ABIN968506)

Bascom, Srinivasan, Nussbaum: "Identification and characterization of golgin-84, a novel Golgi integral membrane protein with a cytoplasmic coiled-coil domain." in: The Journal of biological chemistry, Vol. 274, Issue 5, pp. 2953-62, (1999) (PubMed).

Target Details for GOLGA5

Target: GOLGA5 (Golgin A5 (GOLGA5))

Alternative Name: Golgin-84

Background: The Golgi apparatus is a complex and dynamic organelle that functions in protein sorting and modification. Numerous structural and regulatory proteins are involved in the budding, docking, and fusion of Golgi-directed vesicles. Golgin-84 is an integral membrane protein associated with the Golgi. Sequence analysis of the C-terminal region of Golgin-84 demonstrates a 14 residue region extending into the lumen of the Golgi, a membrane insertion sequence, and a Golgi retention signal. The large N-terminal cytoplasmic region contains a coiled-coil domain that is required for dimerization and two leucine zipper domains. Golgin-84 is ubiquitously expressed, but is abundant in testis. Golgin-84 has sequence homology to coiled-coil containing myosin family members and to several other Golgi proteins, including trans-Golgi p230 and gigantin. Trans-Golgi p230 has been implicated in the biogenesis of specific Golgi vesicles, while gigantin is implicated in the tethering of non-clathrin coated vesicles and in the anchoring of adjacent Golgi cisternae. Thus, Golgin-84 may have similar roles in vesicle biogenesis, docking, and fusion at the Golgi interface.

Molecular Weight: 84 kDa