IGFBP3 antibody (AA 101-210)

Catalog No. ABIN968565

This anti-IGFBP3 antibody is a Mouse Monoclonal antibody detecting IGFBP3 in WB and IF. Suitable for Human. This Primary Antibody has been cited in 4+ publications.

Quick Overview for IGFBP3 antibody (AA 101-210) (ABIN968565)

Target: IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IGFBP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 4-IGFBP

Product Details anti-IGFBP3 Antibody

Binding Specificity: AA 101-210

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human IGFBP-3 aa. 101-210

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-IGFBP3 antibody (ABIN968565)

Campbell, Durham, Hayes, Suwanichkul, Powell: "Insulin-like growth factor-binding protein-3 binds fibrinogen and fibrin." in: The Journal of biological chemistry, Vol. 274, Issue 42, pp. 30215-21, (1999) (PubMed).

Janosi, Firth, Bond, Baxter, Delhanty: "N-Linked glycosylation and sialylation of the acid-labile subunit. Role in complex formation with insulin-like growth factor (IGF)-binding protein-3 and the IGFs." in: The Journal of biological chemistry, Vol. 274, Issue 9, pp. 5292-8, (1999) (PubMed).

Shang, Baumrucker, Green: "Signal relay by retinoic acid receptors alpha and beta in the retinoic acid-induced expression of insulin-like growth factor-binding protein-3 in breast cancer cells." in: The Journal of biological chemistry, Vol. 274, Issue 25, pp. 18005-10, (1999) (PubMed).

Cubbage, Suwanichkul, Powell: "Insulin-like growth factor binding protein-3. Organization of the human chromosomal gene and demonstration of promoter activity." in: The Journal of biological chemistry, Vol. 265, Issue 21, pp. 12642-9, (1990) (PubMed).

Target Details for IGFBP3

Target: IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))

Alternative Name: IGFBP-3

Background: Insulin-like growth factors (IGF) I and II are peptide hormones that regulate cellular proliferation and differentiation. Most circulating IGFs exist in 130-150 kDa ternary complexes containing IGF-Binding Protein-3 (IGFBP-3) and an 85 kDa glycoprotein, the acid-labile subunit (ALS). IGFBP-3 is one of six IGFBPs that, by binding IGF peptides, prolong their half life and maintain the IGF reservoir. IGFBP-3 is found in body fluids as multiple 40-50 kDa forms as a result of differential glycosylation. The expression of IGFBP-3 in many tissues suggests that it locally modulates the autocrine/paracrine action of IGF peptides. IGFBP-3 binding to fibrinogen may be important for wound healing, since this concentrates IGF-I at wound sites and lowers the affinity of IGF-I for IGFBP-3. In addition, retinoic acid-induced IGFBP-3 expression inhibits the growth promoting effects of IGF-I in breast cancer cells. This may link the retinoid and IGF systems in cell growth regulation and explain how the loss of retinoic acid receptor beta leads to breast cancer progression. Thus, IGFBP-3 maintenance and regulation of IGF activity in various tissues may have diverse physiological roles.
Synonyms: IGF-Binding Protein-3

Molecular Weight: 40 & 44 kDa

Pathways: Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding