Thyroid Hormone Receptor Interactor 11 (TRIP11) (AA 159-365) antibody

Catalog No. ABIN968639

The Mouse Monoclonal anti- antibody has been validated for WB and IF. It is suitable to detect in samples from Human. There are 3+ publications available.

Quick Overview for Thyroid Hormone Receptor Interactor 11 (TRIP11) (AA 159-365) antibody (ABIN968639)

Target: Thyroid Hormone Receptor Interactor 11 (TRIP11)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 15-GMAP

Product Details

Binding Specificity: AA 159-365

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human GMAP-210 aa. 159-365

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References

Chen, Chen, Chen, Sharp, Lee: "Thyroid hormone, T3-dependent phosphorylation and translocation of Trip230 from the Golgi complex to the nucleus." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 8, pp. 4443-8, (1999) (PubMed).

Infante, Ramos-Morales, Fedriani, Bornens, Rios: "GMAP-210, A cis-Golgi network-associated protein, is a minus end microtubule-binding protein." in: The Journal of cell biology, Vol. 145, Issue 1, pp. 83-98, (1999) (PubMed).

Chang, Chen, Chen, Chou, Chen, Ma, Yang-Feng, Leng, Tsai, OMalley, Lee: "A thyroid hormone receptor coactivator negatively regulated by the retinoblastoma protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 17, pp. 9040-5, (1997) (PubMed).

Target Details

Target: Thyroid Hormone Receptor Interactor 11 (TRIP11)

Alternative Name: GMAP-210

Background: Trip230 was identified as a protein that interacts with retinoblastoma protein (Rb) and the thyroid hormone receptor (TR). The structure of Trip230 includes five coiled-coil segments separated by non-helical linkers, N-terminal and C-terminal leucine zipper domains, and multiple phosphorylation sites. Trip230 is ubiquitously expressed and localized to the Golgi. During cell cycle progression, a significant portion of Trip230 translocates to the nucleus. In addition, activation of TR with thyroid hormone (T3) leads to phosphorylation of Trip230, as well as Trip230 translocation from the Golgi to the nucleus. Interestingly, Trip230 has also been identified as a Golgi microtubule-associated protein of 210 kDa (GMAP-210). In vitro , GMAP-210 can bind to the minus end of alpha-tubulin and gamma-tubulin via its C-terminal region, while the N-terminal region is involved in Golgi binding. Overexpression of GMAP-210 leads to enlargement of the Golgi apparatus and alterations in the microtubule cytoskeleton. Thus, GMAP-210/Trip230 is thought to function both as a TR coactivator and as a microtubule-binding protein that anchors the Golgi to the microtubule cytoskeleton.
Synonyms: Trip230

Molecular Weight: 210 kDa