LIM Domain Kinase 1 antibody (AA 232-333)

Catalog No. ABIN968654

The Mouse Monoclonal anti-LIM Domain Kinase 1 antibody has been validated for WB and IF. It is suitable to detect LIM Domain Kinase 1 in samples from Human, Mouse and Rat. There are 3+ publications available.

Quick Overview for LIM Domain Kinase 1 antibody (AA 232-333) (ABIN968654)

Target: LIM Domain Kinase 1 (LIMK1)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This LIM Domain Kinase 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 42-LIMK1

Product Details anti-LIM Domain Kinase 1 Antibody

Binding Specificity: AA 232-333

Cross-Reactivity: Rat (Rattus), Mouse (Murine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human LIMK1 aa. 232-333

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968546, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-LIM Domain Kinase 1 antibody (ABIN968654)

Ohashi, Nagata, Maekawa, Ishizaki, Narumiya, Mizuno: "Rho-associated kinase ROCK activates LIM-kinase 1 by phosphorylation at threonine 508 within the activation loop." in: The Journal of biological chemistry, Vol. 275, Issue 5, pp. 3577-82, (2000) (PubMed).

Edwards, Gill: "Structural features of LIM kinase that control effects on the actin cytoskeleton." in: The Journal of biological chemistry, Vol. 274, Issue 16, pp. 11352-61, (1999) (PubMed).

Frangiskakis, Ewart, Morris, Mervis, Bertrand, Robinson, Klein, Ensing, Everett, Green, Pröschel, Gutowski, Noble, Atkinson, Odelberg, Keating: "LIM-kinase1 hemizygosity implicated in impaired visuospatial constructive cognition." in: Cell, Vol. 86, Issue 1, pp. 59-69, (1996) (PubMed).

Target Details for LIM Domain Kinase 1

Target: LIM Domain Kinase 1 (LIMK1)

Alternative Name: LIMK1

Background: Two LIM motif-containing protein kinases (LIMK) have been identified, LIMK1 and LIMK2. These kinases contain two N-terminal LIM domains, a central PDZ domain, and a C-terminal Ser/Thr kinase domain. LIMK1 is highly expressed in brain, heart, and skeletal muscle, while LIMK2 exhibits the highest expression in placenta, liver, lung, kidney, and pancreas. LIMK1 is localized to the actin cytoskeleton and phosphorylates the actin binding/depolymerizing factor, cofilin. During Rho-induced neurite retraction, activation of ROCK leads to LIMK1 activation via phosphorylation at Thr508. In COS-7 cells, disruption of the second LIM domain or the PDZ domain increases LIMK1-induced aggregation of the actin cytoskeleton. In addition, a 32 kDa splice variant that contains only the N-terminus (dLIMK1) suppresses LIMK1 activity by interaction with the C- terminal kinase domain. In humans, deletion of LIMK1 has been implicated in Williams syndrome, a disorder that produces a distinct cognitive profile and vascular disease. Thus, LIMK1, and its splice variant dLIMK1, are thought to have important roles in the regulation of the actin cytoskeleton in a wide varitey of tissues.

Molecular Weight: 72 kDa

Pathways: Caspase Cascade in Apoptosis, Regulation of Cell Size, CXCR4-mediated Signaling Events