Sortilin 1 antibody (AA 300-422)

Catalog No. ABIN968738

The Mouse Monoclonal anti-Sortilin 1 antibody has been validated for WB and IF. It is suitable to detect Sortilin 1 in samples from Human, Rat, Mouse, Dog and Chicken. There are 3+ publications available.

Quick Overview for Sortilin 1 antibody (AA 300-422) (ABIN968738)

Target: Sortilin 1 (SORT1)

Reactivity: Human, Rat, Mouse, Dog, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Sortilin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 48-Neurotensin Receptor 3

Product Details anti-Sortilin 1 Antibody

Binding Specificity: AA 300-422

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Dog (Canine), Chicken

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Neurotensin Receptor 3 aa. 300-422

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968700, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-Sortilin 1 antibody (ABIN968738)

Nielsen, Jacobsen, Olivecrona, Gliemann, Petersen: "Sortilin/neurotensin receptor-3 binds and mediates degradation of lipoprotein lipase." in: The Journal of biological chemistry, Vol. 274, Issue 13, pp. 8832-6, (1999) (PubMed).

Mazella, Zsürger, Navarro, Chabry, Kaghad, Caput, Ferrara, Vita, Gully, Maffrand, Vincent: "The 100-kDa neurotensin receptor is gp95/sortilin, a non-G-protein-coupled receptor." in: The Journal of biological chemistry, Vol. 273, Issue 41, pp. 26273-6, (1998) (PubMed).

Morris, Ross, Lane, Moestrup, Petersen, Keller, Lienhard: "Sortilin is the major 110-kDa protein in GLUT4 vesicles from adipocytes." in: The Journal of biological chemistry, Vol. 273, Issue 6, pp. 3582-7, (1998) (PubMed).

Target Details for Sortilin 1

Target: Sortilin 1 (SORT1)

Alternative Name: Neurotensin Receptor 3

Background: Neurotensin (NT) is a neuropeptide that modulates dopaminergic transmission, triggers analgesic responses, induces hypotension, decreases gastric acid secretion, and activates lipid digestion. NT is a ligand for NT receptors, which include both G-protein coupled and non-G-protein coupled receptors. Neurotensin Receptor 3 (NTR3) is a non-G-protein coupled receptor that has a lumenal domain homologous to sorting proteins, and a short cytoplasmic tail homologous to mannose-6-phosphate/IGF-II receptor. NTR3 was also identified as sortilin/gp95, a component of GLUT4 vesicles in adipocytes that has been implicated in lipoprotein lipase degradation. NTR3 mRNA is expressed in brain, skeletal muscle, heart, and adipocytes. NTR3 mature protein has 44 N-terminal amino acid residues cleaved off, which may facilitate ligand binding to the receptor. Cellular localization of NTR3 is in the Golgi compartment and vesicles, as well as on the cell surface. In addition to roles in neuropeptide and lipoprotein degradation, NTR3 may also be involved in receptor protein sorting. Thus, NTR3 is a multifunctional transmembrane protein that acts as both a intracellular sorting receptor and extracellular ligand-binding receptor.
Synonyms: NTR3

Molecular Weight: 95 kDa

Pathways: Neurotrophin Signaling Pathway